قبل أن تبدأ <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> وينبغي أن تزرع في كل الثقافات 25 درجة مئوية. </li><li style=";text-align:right;direction:rtl"> قد تكون على استعداد HL3.1 العازلة تشريح مقدما. اتخاذ قسامة 50 مل من HL3.1 وابقائه على الجليد. </li><li style=";text-align:right;direction:rtl"> تحضير 15 مل من فورمالديهايد الطازجة بنسبة 3.5 ٪ في HL3.1. يبقيه على الجليد. </li></ol> اليرقات تشريح <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> وضع قطرة من HL3.1 الباردة على لوحة التشريح. هذا وسوف تبقي الحيوان من التجفيف وصعق الحيوان مما يسهل التعامل معها. </li><li style=";text-align:right;direction:rtl"> باستخدام ملقط طويل ، حدد تجول اليرقة طور مرحلي الثالث ووضعه في قطرة من HL3.1. </li><li style=";text-align:right;direction:rtl"> أولا ، استخدام الملقط قصيرة لاستيعاب دبوس minutien. وضع دبوس بين spiracles الخلفي. والحيوان وعادة ما يحاول الزحف بعيدا عن دبوس تمتد نفسها بنفسها ، وجعل من الأسهل بالنسبة لك لوضع دبوس في رأسه بشكل مباشر من يرقة بالقرب من السنانير الفم. تمتد طوليا من أصل الحيوان. هذا سيساعدك على أقصى قدر من جدار الجسم يتعرض يمكنك تحقيق خلال عدة قطاعات. ملاحظة : إذا اخترت دبوس أول رئيس والحيوان قد التفاف حول جسمها دبوس أو سوط الهيئة ذهابا وإيابا مما يجعل من الصعب دبوس. يمكنك مواجهة هذا عادة عن طريق إزالة HL3.1 ووضع قطرة باردة جديدة مذهلة الحيوان مرة أخرى. لا يمكن للعالم أيضا ضبطاء مجرد الاستيلاء على الحيوان والاحتفاظ بها في مكان. </li><li style=";text-align:right;direction:rtl"> مقص الربيع باستخدام إجراء شق أفقي الأمامي لمجرد دبوس الخلفي على الجانب الظهري من اليرقة. مكان واحد شفرة المقص في شق وجعل قطع رأسي على طول خط الوسط ظهري باتجاه نهاية منقاري من اليرقة. على المنصة من الحيوان جعل شقوق الأفقية إلى اليسار واليمين من دبوس. ملاحظة : النتيجة النهائية يجب أن تبدو وكأنها أنا على الجانب الظهري لليرقة على طول محور rostrocaudal. </li><li style=";text-align:right;direction:rtl"> إزالة الأجهزة. تبدأ من خلال وضع العديد من نقاط إضافية HL3.1 على يرقة. وهذا سيمكن أجهزة لتعويم ما يصل إلى خارج الجسم مما يسمح لك لإزالتها بسهولة أكبر بكثير. أولا ، إزالة نظام القصبة الهوائية. ثانيا ، استخدام ملقط لانتزاع ما تبقى من الأجهزة وإزالتها. </li><li style=";text-align:right;direction:rtl"> خلال الخطوة 3 جعلتك رفرف رفرف اليسار واليمين في جدار الجسم. دبوس اللوحات في ترتيب عقارب الساعة مع التأكد من امتداد جدار الجسم أفقيا ورأسيا. </li></ol> تثبيت الإصلاح في كل حيوان الفورمالديهايد بنسبة 3.5 ٪ في HL3.1 لمدة 25 دقيقة. غسل الحيوانات مرتين في HL3.1 لمدة 5 دقائق. تخزين إزالة المسامير ونقل اليرقات إلى أنبوب 1.5 مل تحتوي على 1X PBS. إذا كنت تخطط لاستخدام صورة NMJ به نيون تنصهر فيها ، يجب أن صورة الحيوانات في غضون يومين. قد قمت بتخزين اليرقات تشريح لمدة تصل إلى أسبوع واحد في 4 درجة مئوية. ممثل النتائج هناك عدة عناصر رئيسية ليرقة ذبابة الفاكهة تشريح بشكل صحيح. أولا ، لا بد من اتخاذ المزيد من الحيطة لا يلحق الضرر العضلات ، وخاصة عضلات 4 و 6 و 7. هذه هي عضلات الأكثر شعبية للدراسة ، وإذا كانت معطوبة تجاهل الإعدادية فيليه وتشريح حيوان آخر. ثانيا ، يجب على المرء أن تأكد من أن يتم تمديد الحد الأقصى الحيوان بحيث يمكن تمييز كل العضلات وNMJ.

<ol>
	<li>Jan, L. Y., Jan, Y. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Properties+of+the+larval+neuromuscular+junction+in+Drosophila+Melanogaster.">Properties of the larval neuromuscular junction in Drosophila Melanogaster.</a> J. Physiol. 262, 189-214 (1976).</li><li>Johansen, J., Halpern, M. E., Johansen, K. M., Keshishian, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stereotypic+morphology+of+glutamatergic+synapses+on+identified+muscle+cells+of+Drosophila+larvae.">Stereotypic morphology of glutamatergic synapses on identified muscle cells of Drosophila larvae.</a> J Neurosci. 9, 710-725 (1989).</li><li>Kesheshian, H., Broadie, K., Chiba, A., Bate, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Drosophila+neuromuscular+junction:+a+model+system+for+studying+synaptic+development+and+function.">The Drosophila neuromuscular junction: a model system for studying synaptic development and function.</a> Annu Rev Neurosci. 19, 545-575 (1996).</li><li>Vactor, D. V., Sink, H., Fambrough, D., Tsoo, R., Goodman, C. S. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Genes+that+control+neuromuscular+specificity+in+Drosophila.">Genes that control neuromuscular specificity in Drosophila.</a> Cell. , 73-1137 (1993).</li><li>Feng, . <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=A+modified+minimal+hemolymph-like+solution,+HL3.1,+for+physiological+recordings+at+the+neuromuscular+junctions+of+normal+and+mutant+Drosophila+larvae.">A modified minimal hemolymph-like solution, HL3.1, for physiological recordings at the neuromuscular junctions of normal and mutant Drosophila larvae.</a> J Neurogenet. 18 (2), 377-402 (2004).</li></ol>

ويمكن استخدام هذه التقنية أظهر تشريح في هذا الفيديو لإعداد يرقات ذبابة الفاكهة لمجموعة متنوعة من التقنيات التجريبية. إذا به بروتين فلوري موجودة ، ويمكن تحميل واليرقات والتقط على الفور. خلاف ذلك ، يمكن أن يؤديها من أجل immunostaining علامة مقصورات متشابك محددة. بالإضاف...

HL3 الاحتياطي تشريح : (مم) 70 كلوريد الصوديوم ، 5 بوكل ، 0.2 CaCl2 ، 20 MgCl2 ، 10 NaHCO3 ، 5 طرهالوز ، 115 السكروز ، وHEPES 5 ، ودرجة الحموضة 7.3.<br /<br /&gt; Sylgard تشريح اللوحة : مزيج بلطف (لتجنب فقاعات) 10:01 في كوب. يصب الخليط في طبق بيتري (أي حجم). SylGard تسمح لعلاج بين عشية وضحاها في حاضنة 37C.

drosophila larval nmj dissection

و<em&gt; ذبابة الفاكهة</em&gt; الوصل العصبي العضلي (NMJ) هو نظام يستخدم نموذج المتبع لدراسة تطوير واللدونة متشابك. استخدام على نطاق واسع<em&gt; ذبابة الفاكهة</em&gt; محرك النظام هو نتيجة لسهولة الوصول إليها عالية. ويمكن تحليل ذلك مع قرار وحيد الخلية. هناك 30 في عضلات hemisegment الذين الترتيب داخل جدار الجسم هامشية معروفة. مجموعه من الخلايا العصبية الحركية 35 نعلق على هذه العضلات في النمط الذي الدقة العالية. استخدام البيولوجيا الجزيئية وعلم الوراثة ، ويمكن للمرء أن خلق حيوانات معدلة وراثيا أو المسوخ. ثم ، يمكن للمرء أن دراسة الآثار التنموية على التشكل وظيفة NMJ. من أجل الوصول إلى NMJ للدراسة ، فمن الضروري أن يشرح بعناية كل يرقة. نحن في هذه المقالة شرح كيفية تشريح صحيح<em&gt; ذبابة الفاكهة</em&gt; اليرقات لدراسة NMJ عن طريق إزالة جميع الأعضاء الداخلية في حين ترك جدار الجسم سليما. هذا الأسلوب هو مناسب لاعداد اليرقات التصوير ، والمناعية ، أو الكهربية.

Watch this Scientific Journal Video about Drosophila Larval NMJ Dissection at JoVE.com

Drosophila Larval NMJ Dissection

هذا البروتوكول يوضح كيفية تشريح<em&gt; ذبابة الفاكهة</em&gt; اليرقات في إعداد و / أو التصوير المناعية من مفترق الطرق العصبية والعضلية.

ذبابة الفاكهة تشريح اليرقات NMJ

The Drosophila neuromuscular junction (NMJ) is an established model system used for the study of synaptic development and plasticity. The widespread use ...

Research

JoVE Journal

Biology

34.6K Views.  Columbia University College of Physicians and Surgeons. هذا البروتوكول يوضح كيفية تشريح ذبابة الفاكهة اليرقات في إعداد و / أو التصوير المناعية من مفترق الطرق العصبية والعضلية.

Video: Drosophila Larval NMJ Dissection

Dissection of Drosophila Ovaries

تشريح المبايض ذبابة الفاكهة

Dissection of Larval CNS in Drosophila Melanogaster

The central nervous system (CNS) of Drosophila larvae is complex and poorly understood.  One way to investigate the CNS is to use immunohistochemistry to examine the expression of various novel and marker proteins.  Staining of whole larvae is impractical because the tough cuticle prevents antibodies from penetrating inside the body cavity.  In order to stain these tissues it is necessary to dissect the animal prior to fixing and staining.  In this article we demonstrate how to dissect Drosophila larvae without damaging the CNS.  Begin by tearing the larva in half with a pair of fine forceps, and then turn the cuticle "inside-out" to expose the CNS.  If the dissection is performed carefully the CNS will remain attached to the cuticle.  We usually keep the CNS attached to the cuticle throughout the fixation and staining steps, and only completely remove the CNS from the cuticle just prior to mounting the samples on glass slides.  We also show some representative images of a larval CNS stained with Eve, a transcription factor expressed in a subset of neurons in the CNS.  The article concludes with a discussion of some of the practical uses of this technique and the potential difficulties that may arise.

In this article we demonstrate how to dissect the central nervous system from third instar Drosophila larvae.

تشريح الجهاز العصبي المركزي اليرقات في Melanogaster ذبابة الفاكهة

The central nervous system (CNS) of Drosophila larvae is complex and poorly understood.  One way to investigate the CNS is to use immunohistochemistry to ...

Dissection of Imaginal Discs from 3rd Instar Drosophila Larvae

This protocol demonstrates the dissection technique used for isolating imaginal discs from drosophila larvae at the 3rd instar stage. Methods for fixing the tissue after saying and removing the wing, leg, and eye discs are demonstrated directly on a microscope slide for subsequent visualization.

تشريح أقراص تخيلي من يرقات ذبابة الفاكهة طور مرحلي 3

Drosophila Larval NMJ Immunohistochemistry

The Drosophila neuromuscular junction (NMJ) is an established model system used for the study of synaptic development and plasticity. The widespread use of the Drosophila motor system is due to its high accessibility. It can be analyzed with single-cell resolution. There are 30 muscles per hemisegment whose arrangement within the peripheral body wall are known. A total of 31 motor neurons attach to these muscles in a pattern that has high fidelity. Using molecular biology and genetics, one can create transgenic animals or mutants. Then, one can study the developmental consequences on the morphology and function of the NMJ. Immunohistochemistry can be used to clearly image the components of the NMJ. In this article, we demonstrate how to use antibody staining to visualize the Drosophila larval NMJ.

Drosophila Larval NMJ Immunohistochemistry

This protocol demonstrates how to perform immunohistochemistry on dissected Drosophila larva.

ذبابة الفاكهة اليرقات NMJ المناعية

Electrophysiological Methods for Recording Synaptic Potentials from the NMJ of Drosophila Larvae

In this video, we describe the electrophysiological methods for recording synaptic transmission at the neuromuscular junction (NMJ) of Drosophila larva. The larval neuromuscular system is a model synapse for the study of synaptic physiology and neurotransmission, and is a valuable research tool that has defined genetics and is accessible to experimental manipulation. Larvae can be dissected to expose the body wall musculature, central nervous system, and peripheral nerves. The muscles of Drosophila and their innervation pattern are well characterized and muscles are easy to access for intracellular recording. Individual muscles can be identified by their location and orientation within the 8 abdominal segments, each with 30 muscles arranged in a pattern that is repeated in segments A2 - A7. Dissected drosophila larvae are thin and individual muscles and bundles of motor neuron axons can be visualized by transillumination1. Transgenic constructs can be used to label target cells for visual identification or for manipulating gene products in specific tissues. In larvae, excitatory junction potentials (EJP&#8217;s) are generated in response to vesicular release of glutamate from the motoneurons at the synapse. In dissected larvae, the EJP can be recorded in the muscle with an intracellular electrode. Action potentials can be artificially evoked in motor neurons that have been cut posterior to the ventral ganglion, drawn into a glass pipette by gentle suction and stimulated with an electrode. These motor neurons have distinct firing thresholds when stimulated, and when they fire simultaneously, they generate a response in the muscle. Signals transmitted across the NMJ synapse can be recorded in the muscles that the motor neurons innervate. The EJP&#8217;s and minature excitatory junction potentials (mEJP&#8217;s) are seen as changes in membrane potential. Electrophysiological responses are recorded at room temperature in modified minimal hemolymph-like solution2 (HL3) that contains 5 mM Mg2+ and 1.5 mM Ca2+. Changes in the amplitude of evoked EJP&#8217;s can indicate differences in synaptic function and structure. Digitized recordings are analyzed for EJP amplitude, mEJP frequency and amplitude, and quantal content.

Here we describe electrophysiological methods for measuring synaptic transmission at the neuromuscular junction of Drosophila larva. Evoked release is initiated artificially by stimulating the motor neuron axons, and transmission through the NMJ can be measured by the postsynaptic response evoked in the muscle.

الكهربية طرق تسجيل الإمكانيات متشابك من يرقات ذبابة الفاكهة من NMJ

In this video, we describe the electrophysiological methods for recording synaptic transmission at the neuromuscular junction (NMJ) of Drosophila larva. ...

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

We present various methods to record cardiac function in the larval Drosophila. The approaches allow heart rate to be measured in unrestrained and restrained whole larvae. For direct control of the environment around the heart another approach utilizes the dissected larvae and removal of the internal organs in order to bathe the heart in desired compounds. The exposed heart also allows membrane potentials to be monitored which can give insight of the ionic currents generated by the myocytes and for electrical conduction along the heart tube. These approaches have various advantages and disadvantages for future experiments that are discussed. The larval heart preparation provides an additional model besides the Drosophila skeletal NMJ to investigate the role of intracellular calcium regulation on cellular function. Learning more about the underlying ionic currents that shape the action potentials in myocytes in various species, one can hope to get a handle on the known ionic dysfunctions associated to specific genes responsible for various diseases in mammals.

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

We present various ways to monitor heart function in the larva of Drosophila for assessing questions dealing with the function of gap junctions, ion channel mutations, modulation of pacemaker activity and pharmacological studies.

رصد وظيفة القلب في اليرقات ذبابة الفاكهة السوداء البطن للدراسات فسيولوجية

We present various methods to record cardiac function in the larval Drosophila. The approaches allow heart rate to be measured in unrestrained  and  ...

Dissection of Oenocytes from Adult Drosophila melanogaster

In Drosophila melanogaster, as in other insects, a waxy layer on the outer surface of the cuticle, composed primarily of hydrocarbon compounds, provides protection against desiccation and other environmental challenges. Several of these cuticular hydrocarbon (CHC) compounds also function as semiochemical signals, and as such mediate pheromonal communications between members of the same species, or in some instances between different species, and influence behavior. Specialized cells referred to as oenocytes are regarded as the primary site for CHC synthesis. However, relatively little is known regarding the involvement of the oenocytes in the regulation of the biosynthetic, transport, and deposition pathways contributing to CHC output. Given the significant role that CHCs play in several aspects of insect biology, including chemical communication, desiccation resistance, and immunity, it is important to gain a greater understanding of the molecular and genetic regulation of CHC production within these specialized cells. The adult oenocytes of D. melanogaster are located within the abdominal integument, and are metamerically arrayed in ribbon-like clusters radiating along the inner cuticular surface of each abdominal segment. In this video article we demonstrate a dissection technique used for the preparation of oenocytes from adult D. melanogaster. Specifically, we provide a detailed step-by-step demonstration of (1) how to fillet prepare an adult Drosophila abdomen, (2) how to identify the oenocytes and discern them from other tissues, and (3) how to remove intact oenocyte clusters from the abdominal integument. A brief experimental illustration of how this preparation can be used to examine the expression of genes involved in hydrocarbon synthesis is included. The dissected preparation demonstrated herein will allow for the detailed molecular and genetic analysis of oenocyte function in the adult fruit fly.

Dissection of Oenocytes from Adult Drosophila melanogaster

In insects, the oenocytes produce cuticular hydrocarbon compounds. These compounds protect against desiccation and facilitate chemical communication. Here we demonstrate a dissection technique used to isolate the oenocytes from adult Drosophila melanogaster, and illustrate how this preparation can be utilized to study genes involved in hydrocarbon synthesis.

تشريح Oenocytes من الكبار ذبابة الفاكهة السوداء البطن</em

In Drosophila melanogaster, as in other insects, a waxy layer on the outer surface of the cuticle, composed primarily of hydrocarbon compounds, provides ...

Dissection and Staining of Drosophila Larval Ovaries

Many organs depend on stem cells for their development during embryogenesis and for maintenance or repair during adult life. Understanding how stem cells form, and how they interact with their environment is therefore crucial for understanding development, homeostasis and disease. The ovary of the fruit fly Drosophila melanogaster has served as an influential model for the interaction of germ line stem cells (GSCs) with their somatic support cells (niche) 1, 2. The known location of the niche and the GSCs, coupled to the ability to genetically manipulate them, has allowed researchers to elucidate a variety of interactions between stem cells and their niches 3-12.
Despite the wealth of information about mechanisms controlling GSC maintenance and differentiation, relatively little is known about how GSCs and their somatic niches form during development. About 18 somatic niches, whose cellular components include terminal filament and cap cells (Figure 1), form during the third larval instar 13-17. GSCs originate from primordial germ cells (PGCs). PGCs proliferate at early larval stages, but following the formation of the niche a subgroup of PGCs becomes GSCs 7, 16, 18, 19. Together, the somatic niche cells and the GSCs make a functional unit that produces eggs throughout the lifetime of the organism.
Many questions regarding the formation of the GSC unit remain unanswered. Processes such as coordination between precursor cells for niches and stem cell precursors, or the generation of asymmetry within PGCs as they become GSCs, can best be studied in the larva. However, a methodical study of larval ovary development is physically challenging. First, larval ovaries are small. Even at late larval stages they are only 100&mu;m across. In addition, the ovaries are transparent and are embedded in a white fat body. Here we describe a step-by-step protocol for isolating ovaries from late third instar (LL3) Drosophila larvae, followed by staining with fluorescent antibodies. We offer some technical solutions to problems such as locating the ovaries, staining and washing tissues that do not sink, and making sure that antibodies penetrate into the tissue. This protocol can be applied to earlier larval stages and to larval testes as well.

Dissection and Staining of Drosophila Larval Ovaries

How niches and stem cells form during development is an important question with practical implications. In the Drosophila ovary, germ line stem cells and their somatic niches form during larval development. This video demonstrates how to dissect, stain and mount female gonads from late third instar (LL3) Drosophila larvae.

تشريح وتلون ذبابة الفاكهة اليرقات المبايض

Many organs depend on stem cells for their development during embryogenesis and for maintenance or repair during adult life. Understanding how stem cells ...

Heart Dissection in Larval, Juvenile and Adult Zebrafish, Danio rerio

Zebrafish have become a beneficial and practical model organism for the study of embryonic heart development (see recent reviews1-6), however, work examining post-embryonic through adult cardiac development has been limited7-10. Examining the changing morphology of the maturing and aging heart are restricted by the lack of techniques available for staging and isolating juvenile and adult hearts. In order to analyze heart development over the fish's lifespan, we dissect zebrafish hearts at numerous stages and photograph them for further analysis11. The morphological features of the heart can easily be quantified and individual hearts can be further analyzed by a host of standard methods. Zebrafish grow at variable rates and maturation correlates better with fish size than age, thus, post-fixation, we photograph and measure fish length as a gauge of fish maturation. This protocol explains two distinct, size dependent dissection techniques for zebrafish, ranging from larvae 3.5mm standard length (SL) with hearts of 100&mu;m ventricle length (VL), to adults, with SL of 30mm and VL 1mm or larger. Larval and adult fish have quite distinct body and organ morphology. Larvae are not only significantly smaller, they have less pigment and each organ is visually very difficult to identify. For this reason, we use distinct dissection techniques.
We used pre-dissection fixation procedures, as we discovered that hearts dissected directly after euthanization have a more variable morphology, with very loose and balloon like atria compared with hearts removed following fixation. The fish fixed prior to dissection, retain in vivo morphology and chamber position (data not shown). In addition, for demonstration purposes, we take advantage of the heart (myocardial) specific GFP transgenic Tg(myl7:GFP)twu34 (12), which allows us to visualize the entire heart and is particularly useful at early stages in development when the cardiac morphology is less distinct from surrounding tissues. Dissection of the heart makes further analysis of the cell and molecular biology underlying heart development and maturation using in situ hybridization, immunohistochemistry, RNA extraction or other analytical methods easier in post-embryonic zebrafish. This protocol will provide a valuable technique for the study of cardiac development maturation and aging.

Heart Dissection in Larval, Juvenile and Adult Zebrafish, Danio rerio

A clear, standardized method for dissection and isolation of the zebrafish heart at multiple developmental stages are described. Annotation and quantification techniques are also discussed.

تشريح القلب في اليرقات ، اسماك الزرد الأحداث والكبار ، دانيو rerio</em

Zebrafish have become a beneficial and practical model organism for the study of embryonic heart development (see recent reviews1-6), however, work ...

Examination of Drosophila Larval Tracheal Terminal Cells by Light Microscopy

Cell shape is critical for cell function. However, despite the importance of cell morphology, little is known about how individual cells generate specific shapes. Drosophila tracheal terminal cells have become a powerful genetic model to identify and elucidate the roles of genes required for generating cellular morphologies. Terminal cells are a component of a branched tubular network, the tracheal system that functions to supply oxygen to internal tissues. Terminal cells are an excellent model for investigating questions of cell shape as they possess two distinct cellular architectures. First, terminal cells have an elaborate branched morphology, similar to complex neurons; second, terminal cell branches are formed as thin tubes and contain a membrane-bound intracellular lumen. Quantitative analysis of terminal cell branch number, branch organization and individual branch shape, can be used to provide information about the role of specific genetic mechanisms in the making of a branched cell. Analysis of tube formation in these cells can reveal conserved mechanisms of tubulogenesis common to other tubular networks, such as the vertebrate vasculature. Here we describe techniques that can be used to rapidly fix, image, and analyze both branching patterns and tube formation in terminal cells within Drosophila larvae. These techniques can be used to analyze terminal cells in wild-type and mutant animals, or genetic mosaics. Because of the high efficiency of this protocol, it is also well suited for genetic, RNAi-based, or drug screens in the Drosophila tracheal system.

Examination of Drosophila Larval Tracheal Terminal Cells by Light Microscopy

Here, we present a method for light microscopy analysis of tracheal terminal cells in Drosophila larvae. This method allows for quick examination of branch and lumen morphology in whole animals and would be useful for analysis of individual mutants or screens for mutations affecting terminal cell development.

فحص<em&gt; ذبابة الفاكهة</em&gt; اليرقات الخلايا الطرفية الرغامي بواسطة المجهر الضوئي

Cell shape is critical for cell function. However, despite the importance of cell morphology, little is known about how individual cells generate specific ...