قبل أن تبدأ <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> إعداد الحلول التالية : PBT (0.1 ٪ X100 تريتون في برنامج تلفزيوني 1X) ، PBTB (0.2 ٪ في جيش صرب البوسنة PBT) ، وPBTN (خ ع 2 ٪ في PBTB). </li><li style=";text-align:right;direction:rtl"> تشريح ذبابة الفاكهة اليرقات. يرجى الاطلاع على ذبابة الفاكهة تشريح NMJ اليرقات. </li></ol> المناعية <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> نقل اليرقات إلى أنبوب 1.5 مل تحتوي على PBT. غسل اليرقات مرتين لمدة 15 دقيقة في PBT. ملاحظة : أن يغسل ، ووضع أنبوب مل 1.5 على خلاط nutator. إزالة السائل مع pippetor واستبدالها السائلة الطازجة. </li><li style=";text-align:right;direction:rtl"> إزالة PBT. يغسل في PBTB مرتين لمدة 30 دقيقة. </li><li style=";text-align:right;direction:rtl"> إزالة PBTB. يغسل في PBTN مرتين لمدة 15 دقيقة. </li><li style=";text-align:right;direction:rtl"> في احتضان الضد الأولية مخففة في PBTN في درجة حرارة الغرفة لمدة 1 ساعة أو عند 4 درجات مئوية خلال الليل. </li><li style=";text-align:right;direction:rtl"> شطف مرتين مع PBTB. ملاحظة : حل لشطف إضافة وإزالة بسرعة. </li><li style=";text-align:right;direction:rtl"> تغسل مرتين لمدة 15 دقيقة في PBTB. </li><li style=";text-align:right;direction:rtl"> يغسل في PBTN لمدة 30 دقيقة. </li><li style=";text-align:right;direction:rtl"> في احتضان الضد الثانوية (والضد الأولية مترافق إذا كنت تستخدم واحد) في PBTN المخفف. </li><li style=";text-align:right;direction:rtl"> تغطي في البربون واحتضان مقابل 1.5 ساعة في درجة حرارة الغرفة. </li><li style=";text-align:right;direction:rtl"> شطف مرتين مع PBTB. </li><li style=";text-align:right;direction:rtl"> تغسل مرتين لمدة 15 دقيقة مع PBTB. </li><li style=";text-align:right;direction:rtl"> الشروع في التركيب. </li></ol> متزايد ملاحظة : في جبل الجلسرين إذا كان سيتم تصوير العينات على الفور. جبل الذهب في إطالة ما اذا كان سيتم تخزين العينات قبل التصوير (لأكثر من أسبوع) ، أو إذا كان يجب التقط عينات عدة مرات. للاستخدام ، وضع زجاجة من إطالة في 65 º C حمام الماء لمدة 2-3 دقائق. يأخذ قسامة من الذهب وإطالة البقاء على على كتلة حرارة 45-50 درجة مئوية في <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> صب محضرات الملون في PBT إلى كوب ووتش. </li><li style=";text-align:right;direction:rtl"> وضع بعض الجلسرين على شريحة زجاجية نظيفة للمعالجة. </li><li style=";text-align:right;direction:rtl"> نقل الحيوانات إلى شريحة المعالجة من قبل انتشالها من الزجاج ووتش زاوية باستخدام ملقط. ضمان أن تكون بشرة الجانب الأسفل. </li><li style=";text-align:right;direction:rtl"> إزالة الرأس والذيل مع شفرة حلاقة جديدة على معالجة شريحة زجاجية. وexacto سكين مع شفرة # 16 يعمل بشكل جيد لهذا الغرض. </li><li style=";text-align:right;direction:rtl"> على شريحة أخرى (تركيب الشريحة) ، وضع قطرة صغيرة من الجلسرين / إطالة وانتشاره مع ملقط نظيفة. </li><li style=";text-align:right;direction:rtl"> تشريح الحيوانات نقل إلى الشريحة المتزايدة التي ميزتها ، مع الحرص على عدم قلب لهم. محاولة تحميل لهم في الصفوف في اتجاه واحد. جبل 6-8 الحيوانات لكل شريحة. </li><li style=";text-align:right;direction:rtl"> انخفاض زلة تغطية على حافة طريق وضع في الجلسرين / إطالة والإفراج عن ببطء. </li><li style=";text-align:right;direction:rtl"> ختم الشريحة مع طلاء الأظافر. علما [لا صورة حتى طلاء الأظافر جافة. هذا عادة ما يستغرق عشر دقائق. للعينات التي شنت في إطالة الذهب ، والسماح للشرائح الجافة لثلاث ساعات على الأقل قبل الختم أو التصوير.] </li></ol>

<ol>
	<li>Jan, L. Y., Jan, Y. N. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Properties+of+the+larval+neuromuscular+junction+in+Drosophila+Melanogaster.">Properties of the larval neuromuscular junction in Drosophila Melanogaster.</a> J. Physiol. 262, 189-214 (1976).</li><li>Johansen, J., Halpern, M. E., Johansen, K. M., Keshishian, H. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Stereotypic+morphology+of+glutamatergic+synapses+on+identified+muscle+cells+of+Drosophila+larvae.">Stereotypic morphology of glutamatergic synapses on identified muscle cells of Drosophila larvae.</a> J Neurosci. 9, 710-725 (1989).</li><li>Kesheshian, H., Broadie, K., Chiba, A., Bate, M. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=The+Drosophila+neuromuscular+junction:+a+model+system+for+studying+synaptic+development+and+function.">The Drosophila neuromuscular junction: a model system for studying synaptic development and function.</a> Annu Rev Neurosci. 19, 545-575 (1996).</li><li>Collins, C. A., DiAntonio, A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Synaptic+development:+insights+from+Drosophila.">Synaptic development: insights from Drosophila.</a> Curr Opin Neurobiol. 17 (1), 35-42 (2007).</li></ol>

المناعية (IHC) أمر حيوي لدراسة البيولوجيا NMJ لأنه يمكن تصور من NMJ. ويتم إنجاز هذا باستخدام الأجسام المضادة التي تعترف غشاء الخلايا العصبية (على سبيل المثال ، HRP) ، وpresynapse (على سبيل المثال ، CSP ، SYT) ، و / أو postsynapse (على سبيل المثال ، DLG). ويمكن أيضا الإشارة الجزيئات والبروتينات ...

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drosophila larval nmj immunohistochemistry

و<em&gt; ذبابة الفاكهة</em&gt; الوصل العصبي العضلي (NMJ) هو نظام يستخدم نموذج المتبع لدراسة تطوير واللدونة متشابك. استخدام على نطاق واسع<em&gt; ذبابة الفاكهة</em&gt; محرك النظام هو نتيجة لسهولة الوصول إليها عالية. ويمكن تحليل ذلك مع قرار وحيد الخلية. هناك 30 في عضلات hemisegment الذين الترتيب داخل جدار الجسم هامشية معروفة. مجموعه من الخلايا العصبية الحركية 31 نعلق على هذه العضلات في النمط الذي الدقة العالية. استخدام البيولوجيا الجزيئية وعلم الوراثة ، ويمكن للمرء أن خلق حيوانات معدلة وراثيا أو المسوخ. ثم ، يمكن للمرء أن دراسة الآثار التنموية على التشكل وظيفة NMJ. ويمكن استخدام المناعية لصورة واضحة لعناصر NMJ. في هذه المقالة ، ونحن لشرح كيفية استخدام تلوين الأجسام المضادة لتصور<em&gt; ذبابة الفاكهة</em&gt; NMJ اليرقات.

Watch this Scientific Journal Video about Drosophila Larval NMJ Immunohistochemistry at JoVE.com

Drosophila Larval NMJ Immunohistochemistry

هذا البروتوكول يوضح كيفية تنفيذ المناعية على يرقة ذبابة الفاكهة تشريح.

ذبابة الفاكهة اليرقات NMJ المناعية

The Drosophila neuromuscular junction (NMJ) is an established model system used for the study of synaptic development and plasticity. The widespread use ...

drosophila-larval-nmj-immunohistochemistry

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JoVE Journal

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Drosophila Larval NMJ Immunohistochemistry

15.4K Views.  Columbia University College of Physicians and Surgeons. هذا البروتوكول يوضح كيفية تنفيذ المناعية على يرقة ذبابة الفاكهة تشريح.

Video: Drosophila Larval NMJ Immunohistochemistry

Dissection of Larval CNS in Drosophila Melanogaster

The central nervous system (CNS) of Drosophila larvae is complex and poorly understood.  One way to investigate the CNS is to use immunohistochemistry to examine the expression of various novel and marker proteins.  Staining of whole larvae is impractical because the tough cuticle prevents antibodies from penetrating inside the body cavity.  In order to stain these tissues it is necessary to dissect the animal prior to fixing and staining.  In this article we demonstrate how to dissect Drosophila larvae without damaging the CNS.  Begin by tearing the larva in half with a pair of fine forceps, and then turn the cuticle "inside-out" to expose the CNS.  If the dissection is performed carefully the CNS will remain attached to the cuticle.  We usually keep the CNS attached to the cuticle throughout the fixation and staining steps, and only completely remove the CNS from the cuticle just prior to mounting the samples on glass slides.  We also show some representative images of a larval CNS stained with Eve, a transcription factor expressed in a subset of neurons in the CNS.  The article concludes with a discussion of some of the practical uses of this technique and the potential difficulties that may arise.

In this article we demonstrate how to dissect the central nervous system from third instar Drosophila larvae.

تشريح الجهاز العصبي المركزي اليرقات في Melanogaster ذبابة الفاكهة

The central nervous system (CNS) of Drosophila larvae is complex and poorly understood.  One way to investigate the CNS is to use immunohistochemistry to ...

Thin Sectioning of Slice Preparations for Immunohistochemistry

Many investigations in neuroscience, as well as other disciplines, involve studying small, yet macroscopic pieces or sections of tissue that have been preserved, freshly removed, or excised but kept viable, as in slice preparations of brain tissue.  Subsequent microscopic studies of this material can be challenging, as the tissue samples may be difficult to handle.  Demonstrated here is a method for obtaining thin cryostat sections of tissue with a thickness that may range from 0.2-5.0 mm.  We routinely cut 400 micron thick Vibratome brain slices serially into 5-10 micron coronal cryostat sections.  The slices are typically first used for electrophysiology experiments and then require microscopic analysis of the cytoarchitecture of the region from which the recordings were observed.  We have constructed a simple device that allows controlled and reproducible preparation and positioning of the tissue slice.  This device consists of a cylinder 5 cm in length with a diameter of 1.2 cm, which serves as a freezing stage for the slice.  A ring snugly slides over the cylinder providing walls around the slice allowing the tissue to be immersed in freezing compound (e.g., OCT).  This is then quickly frozen with crushed dry ice and the resulting  wafer  can be position easily for cryostat sectioning.  Thin sections can be thaw-mounted onto coated slides to allow further studies to be performed, such as various staining methods, in situ hybridization, or immunohistochemistry, as demonstrated here.

The present method allows reproducible cryostat sectioning of small, difficult-to-manage, tissue pieces, such as biopsies and brain slices. We utilize a simple aluminum freezing stage to facilitate handling of tissue and a standard cryostat to routinely produce 5-10 micron serial sections from 400 micron thick brain slices.

Sectioning رقيقة من الاستعدادات لشريحة المناعية

Many investigations in neuroscience, as well as other disciplines, involve studying small, yet macroscopic pieces or sections of tissue that have been ...

Drosophila Larval NMJ Dissection

The Drosophila neuromuscular junction (NMJ) is an established model system used for the study of synaptic development and plasticity. The widespread use of the Drosophila motor system is due to its high accessibility. It can be analyzed with single-cell resolution. There are 30 muscles per hemisegment whose arrangement within the peripheral body wall are known. A total of 35 motor neurons attach to these muscles in a pattern that has high fidelity. Using molecular biology and genetics, one can create transgenic animals or mutants. Then, one can study the developmental consequences on the morphology and function of the NMJ. In order to access the NMJ for study, it is necessary to carefully dissect each larva. In this article we demonstrate how to properly dissect Drosophila larvae for study of the NMJ by removing all internal organs while leaving the body wall intact. This technique is suitable to prepare larvae for imaging, immunohistochemistry, or electrophysiology.

This protocol demonstrates how to dissect Drosophila larvae in preparation for immunohistochemistry and/or imaging of the neuromuscular junction.

ذبابة الفاكهة تشريح اليرقات NMJ

Electrophysiological Methods for Recording Synaptic Potentials from the NMJ of Drosophila Larvae

In this video, we describe the electrophysiological methods for recording synaptic transmission at the neuromuscular junction (NMJ) of Drosophila larva. The larval neuromuscular system is a model synapse for the study of synaptic physiology and neurotransmission, and is a valuable research tool that has defined genetics and is accessible to experimental manipulation. Larvae can be dissected to expose the body wall musculature, central nervous system, and peripheral nerves. The muscles of Drosophila and their innervation pattern are well characterized and muscles are easy to access for intracellular recording. Individual muscles can be identified by their location and orientation within the 8 abdominal segments, each with 30 muscles arranged in a pattern that is repeated in segments A2 - A7. Dissected drosophila larvae are thin and individual muscles and bundles of motor neuron axons can be visualized by transillumination1. Transgenic constructs can be used to label target cells for visual identification or for manipulating gene products in specific tissues. In larvae, excitatory junction potentials (EJP&#8217;s) are generated in response to vesicular release of glutamate from the motoneurons at the synapse. In dissected larvae, the EJP can be recorded in the muscle with an intracellular electrode. Action potentials can be artificially evoked in motor neurons that have been cut posterior to the ventral ganglion, drawn into a glass pipette by gentle suction and stimulated with an electrode. These motor neurons have distinct firing thresholds when stimulated, and when they fire simultaneously, they generate a response in the muscle. Signals transmitted across the NMJ synapse can be recorded in the muscles that the motor neurons innervate. The EJP&#8217;s and minature excitatory junction potentials (mEJP&#8217;s) are seen as changes in membrane potential. Electrophysiological responses are recorded at room temperature in modified minimal hemolymph-like solution2 (HL3) that contains 5 mM Mg2+ and 1.5 mM Ca2+. Changes in the amplitude of evoked EJP&#8217;s can indicate differences in synaptic function and structure. Digitized recordings are analyzed for EJP amplitude, mEJP frequency and amplitude, and quantal content.

Here we describe electrophysiological methods for measuring synaptic transmission at the neuromuscular junction of Drosophila larva. Evoked release is initiated artificially by stimulating the motor neuron axons, and transmission through the NMJ can be measured by the postsynaptic response evoked in the muscle.

الكهربية طرق تسجيل الإمكانيات متشابك من يرقات ذبابة الفاكهة من NMJ

In this video, we describe the electrophysiological methods for recording synaptic transmission at the neuromuscular junction (NMJ) of Drosophila larva. ...

Historical View and Physiology Demonstration at the NMJ of the Crayfish Opener Muscle

Here we present some of the key important discoveries made with the opener neuromuscular (NMJ) preparation of crustaceans and illustrate that there is still much to learn from this model preparation. In understanding the history one can appreciate why even today this NMJ still offers a rich playground to address questions regarding pre- and post-synaptic function and plasticity. The viability and ease of access to the terminal for intracellular as well as extracellular electrophysiology and imaging are significant advantages. The mechanisms behind the modulation of vesicular kinetics and fusion within the high- and low-output terminals are begging for investigation. The preparation also offers a testable model system for computational assessments and manipulations to examine key variables in theoretical models of synaptic function, for example calcium dynamics during short-term facilitation. The synaptic complexity of active zone and statistical nature of quantal release is also an open area for future investigation both experimentally and computationally.

The opener muscle of the crayfish leg is presented for its historical importance and experimental versatility in muscle phenotype, synaptic physiology and plasticity.

عرض تاريخي والتظاهر في فسيولوجيا NMJ من جراد البحر العضلات فتاحة

Here we present some of the key important discoveries made with the opener neuromuscular (NMJ) preparation of crustaceans and illustrate that there is ...

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

We present various methods to record cardiac function in the larval Drosophila. The approaches allow heart rate to be measured in unrestrained and restrained whole larvae. For direct control of the environment around the heart another approach utilizes the dissected larvae and removal of the internal organs in order to bathe the heart in desired compounds. The exposed heart also allows membrane potentials to be monitored which can give insight of the ionic currents generated by the myocytes and for electrical conduction along the heart tube. These approaches have various advantages and disadvantages for future experiments that are discussed. The larval heart preparation provides an additional model besides the Drosophila skeletal NMJ to investigate the role of intracellular calcium regulation on cellular function. Learning more about the underlying ionic currents that shape the action potentials in myocytes in various species, one can hope to get a handle on the known ionic dysfunctions associated to specific genes responsible for various diseases in mammals.

Monitoring Heart Function in Larval Drosophila melanogaster for Physiological Studies

We present various ways to monitor heart function in the larva of Drosophila for assessing questions dealing with the function of gap junctions, ion channel mutations, modulation of pacemaker activity and pharmacological studies.

رصد وظيفة القلب في اليرقات ذبابة الفاكهة السوداء البطن للدراسات فسيولوجية

We present various methods to record cardiac function in the larval Drosophila. The approaches allow heart rate to be measured in unrestrained  and  ...

Dissection and Staining of Drosophila Larval Ovaries

Many organs depend on stem cells for their development during embryogenesis and for maintenance or repair during adult life. Understanding how stem cells form, and how they interact with their environment is therefore crucial for understanding development, homeostasis and disease. The ovary of the fruit fly Drosophila melanogaster has served as an influential model for the interaction of germ line stem cells (GSCs) with their somatic support cells (niche) 1, 2. The known location of the niche and the GSCs, coupled to the ability to genetically manipulate them, has allowed researchers to elucidate a variety of interactions between stem cells and their niches 3-12.
Despite the wealth of information about mechanisms controlling GSC maintenance and differentiation, relatively little is known about how GSCs and their somatic niches form during development. About 18 somatic niches, whose cellular components include terminal filament and cap cells (Figure 1), form during the third larval instar 13-17. GSCs originate from primordial germ cells (PGCs). PGCs proliferate at early larval stages, but following the formation of the niche a subgroup of PGCs becomes GSCs 7, 16, 18, 19. Together, the somatic niche cells and the GSCs make a functional unit that produces eggs throughout the lifetime of the organism.
Many questions regarding the formation of the GSC unit remain unanswered. Processes such as coordination between precursor cells for niches and stem cell precursors, or the generation of asymmetry within PGCs as they become GSCs, can best be studied in the larva. However, a methodical study of larval ovary development is physically challenging. First, larval ovaries are small. Even at late larval stages they are only 100&mu;m across. In addition, the ovaries are transparent and are embedded in a white fat body. Here we describe a step-by-step protocol for isolating ovaries from late third instar (LL3) Drosophila larvae, followed by staining with fluorescent antibodies. We offer some technical solutions to problems such as locating the ovaries, staining and washing tissues that do not sink, and making sure that antibodies penetrate into the tissue. This protocol can be applied to earlier larval stages and to larval testes as well.

Dissection and Staining of Drosophila Larval Ovaries

How niches and stem cells form during development is an important question with practical implications. In the Drosophila ovary, germ line stem cells and their somatic niches form during larval development. This video demonstrates how to dissect, stain and mount female gonads from late third instar (LL3) Drosophila larvae.

تشريح وتلون ذبابة الفاكهة اليرقات المبايض

Many organs depend on stem cells for their development during embryogenesis and for maintenance or repair during adult life. Understanding how stem cells ...

Drosophila Pupal Abdomen Immunohistochemistry

The Drosophila pupal abdomen is an established model system for the study of epithelial morphogenesis and the development of sexually dimorphic morphologies 1-3. During pupation, which spans approximately 96 hours (at 25 &deg;C), proliferating populations of imaginal cells replace the larval epidermis to generate the adult abdominal segments. These imaginal cells, born during embryogenesis, exist as lateral pairs of histoblast nests in each abdominal segment of the larvae. Four pairs of histoblast nests give rise to the adult dorsal cuticle (anterior and posterior dorsal nests), the ventral cuticle (ventral nests) and the spiracles associated with each segment (spiracle nests) 4. Upon puparation, these diploid cells (distinguishable by size from the larger polyploid larval epidermal cells- LECs) begin a stereotypical process of proliferation, migration and replacement of the LECs. Various molecular and genetic tools can be employed to investigate the contributions of genetic pathways involved in morphogenesis of the adult abdomen. Ultimate adult phenotypes are typically analyzed following dissection of adult abdominal cuticles. However, investigation of the underlying molecular processes requires immunohistochemical analyses of the pupal epithelium, which present unique challenges. Temporally dynamic morphogenesis and the interactions of two distinct epithelial populations (larval and imaginal) generate a fragile tissue prone to excessive cell loss during dissection and subsequent processing. We have developed methods of dissection, fixation, mounting and imaging of the Drosophila pupal abdominem epithelium for immunohistochemical studies that generate consistent high quality samples suitable for confocal or standard fluorescent microscopy.

Drosophila Pupal Abdomen Immunohistochemistry

Antibody staining of the Drosophila pupae can enhance genetic analyses of adult abdominal developmental genetics. We present our protocol for dissection, fixation and antibody staining of staged Drosophila pupal abdomen.

ذبابة الفاكهة المناعية العذراء البطن

The Drosophila pupal abdomen is an established model system for the study of epithelial morphogenesis and the development of sexually dimorphic ...

Examination of Drosophila Larval Tracheal Terminal Cells by Light Microscopy

Cell shape is critical for cell function. However, despite the importance of cell morphology, little is known about how individual cells generate specific shapes. Drosophila tracheal terminal cells have become a powerful genetic model to identify and elucidate the roles of genes required for generating cellular morphologies. Terminal cells are a component of a branched tubular network, the tracheal system that functions to supply oxygen to internal tissues. Terminal cells are an excellent model for investigating questions of cell shape as they possess two distinct cellular architectures. First, terminal cells have an elaborate branched morphology, similar to complex neurons; second, terminal cell branches are formed as thin tubes and contain a membrane-bound intracellular lumen. Quantitative analysis of terminal cell branch number, branch organization and individual branch shape, can be used to provide information about the role of specific genetic mechanisms in the making of a branched cell. Analysis of tube formation in these cells can reveal conserved mechanisms of tubulogenesis common to other tubular networks, such as the vertebrate vasculature. Here we describe techniques that can be used to rapidly fix, image, and analyze both branching patterns and tube formation in terminal cells within Drosophila larvae. These techniques can be used to analyze terminal cells in wild-type and mutant animals, or genetic mosaics. Because of the high efficiency of this protocol, it is also well suited for genetic, RNAi-based, or drug screens in the Drosophila tracheal system.

Examination of Drosophila Larval Tracheal Terminal Cells by Light Microscopy

Here, we present a method for light microscopy analysis of tracheal terminal cells in Drosophila larvae. This method allows for quick examination of branch and lumen morphology in whole animals and would be useful for analysis of individual mutants or screens for mutations affecting terminal cell development.

فحص<em&gt; ذبابة الفاكهة</em&gt; اليرقات الخلايا الطرفية الرغامي بواسطة المجهر الضوئي

Cell shape is critical for cell function. However, despite the importance of cell morphology, little is known about how individual cells generate specific ...

Sonication-facilitated Immunofluorescence Staining of Late-stage Embryonic and Larval Drosophila Tissues In Situ

Studies performed in Drosophila melanogaster embryos and larvae provide crucial insight into developmental processes such as cell fate specification and organogenesis. Immunostaining allows for the visualization of developing tissues and organs. However, a protective cuticle that forms at the end of embryogenesis prevents permeation of antibodies into late-stage embryos and larvae. While dissection prior to immunostaining is regularly used to analyze Drosophila larval tissues, it proves inefficient for some analyses because small tissues may be difficult to locate and isolate. Sonication provides an alternative to dissection in larval Drosophila immunostaining protocols. It allows for quick, simultaneous processing of large numbers of late-stage embryos and larvae and maintains in situ morphology. After fixation in formaldehyde, a sample is sonicated. Sample is then subjected to immunostaining with antigen-specific primary antibodies and fluorescently labeled secondary antibodies to visualize target cell types and specific proteins via fluorescence microscopy. During the process of sonication, proper placement of a sonicating probe above the sample, as well as the duration and intensity of sonication, is critical. Additonal minor modifications to standard immunostaining protocols may be required for high quality stains. For antibodies with low signal to noise ratio, longer incubation times are typically necessary. As a proof of concept for this sonication-facilitated protocol, we show immunostains of three tissue types (testes, ovaries, and neural tissues) at a range of developmental stages.

Sonication-facilitated Immunofluorescence Staining of Late-stage Embryonic and Larval Drosophila Tissues In Situ

Immunostaining is an effective technique for visualizing specific cell types and proteins within tissues. By utilizing sonication, the protocol described here alleviates the need to dissect Drosophila melanogaster tissues from late-stage embryos and larvae before immunostaining. We provide an efficient methodology for the immunostaining of formaldehyde-fixed whole mount larvae.

-سهلت صوتنة المناعي تلطيخ المرحلة الجنينية في وقت متأخر من اليرقات و<em&gt; ذبابة الفاكهة</em&gt; الأنسجة<em&gt; في الموقع</em

Studies performed in Drosophila melanogaster embryos and larvae provide crucial insight into developmental processes such as cell fate specification and ...