We would like to thank the whole Service of Neuroradiology. We would like to thank Lis Prussen for her work in the library.

ملاحظة: هذا البروتوكول يتبع المبادئ التوجيهية للمركز HOSPITALIER دي لوكسمبورغ في لوكسمبورغ. 1. إعداد إنتشار العضلة الشادة التصوير لمسار البصرية لجراحة المخ والأعصاب والمتابعة <ol style="margin-left: 40px;;text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> إجراء المسح بالرنين المغناطيسي قبل الجراحة المحورية بدقة باستخدام 32 الاتجاهات التدرج واحدة B0-صورة يوم واحد على الأقل. البقاء على اتصال وثيق مع وحدة التصوير الشعاعي العصبي في أي لحظة. ملاحظة: كن واضحا للneuroradiologist أن الصور بعد الجراحة هي نفسها التي قبل العملية. </li><li style=";text-align:right;direction:rtl"> باستخدام التصوير بالرنين المغناطيسي 3 تسلا، إجراء 3DT1 مرجح وزارة التجارة والصناعة تسلسل بالاشعة. إجراء تسلسل 3DT1 مرجح بعد الجراحة أيضا. </li></ol> 2. عن طريق محطة التخطيط <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> نقل البيانات مسح المرجحة T2، 3DT1 مرجح وزارة التجارة والصناعة تسلسل إلى التصوير الرقمي والاتصالات في الطب (DICOM). هذا الإجراء يستغرق فترة تصل إلى 7 دقائق. ملاحظة: دون &#39;ر وقف الإجراء befoبعد إعادة نقل جميع متواليات. فمن الممكن لوالتوقف عن الاستمرار في وقت لاحق اعتمادا على تاريخ الجراحة. </li><li style=";text-align:right;direction:rtl"> فتح برنامج نظام الملاحة الجراحية. انقر على ملف ثم على استيراد DICOM. كرر هذا الإجراء ثلاث مرات لجميع متواليات المذكورة أعلاه. <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> انقر فوق إضافة إلى عرضها. إضافة تسلسل كل على حدة. دون &#39;ر محاولة المضي قدما في عرض. </li></ol></li><li style=";text-align:right;direction:rtl"> انقر على أدوات. مفتوحة زارة التجارة والصناعة إعداد العضلة الشادة. مراقبة نافذة جديدة في وسط الشاشة. </li><li style=";text-align:right;direction:rtl"> استكمال الخطوات الأربعة التالية. <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> أداء الواجب التدرج كخطوة أولى. <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> ب تغيير القيمة من 1،000 إلى 800 ق / مم ² على اليمين السفلي من النافذة. </li><li style=";text-align:right;direction:rtl"> ضبط عتبة على الجانب الأيمن العلوي من النافذة. القيام بذلك يدويا ببساطة عن طريق كتابة رقم أو تحريك المؤشر. 20 قد تكون قيمة مقبولة. ومن تجربة شخصية وليس إلزاميا. </li></ol></li><li style=";text-align:right;direction:rtl"> أداء التسجيل التدرج والخطوة الثانية. <ol style=";text-align:right;direction:rtl"> &lt;<html> li&gt; انقر على زر جميع السيارات. هذا الإجراء يستغرق فترة تصل إلى 5 دقائق. </li><li style=";text-align:right;direction:rtl"> انقر على التحقق من جميع التسجيلات. دون التأكد من التسجيلات أنه ليس من الممكن أن يستمر. </li></ol></li><li style=";text-align:right;direction:rtl"> أداء Coregistration كخطوة ثالثة. <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> Coregister MR1 والصور MR2 B0 يدويا. في النهاية تحقق جميع التسجيلات. ملاحظة: من الممكن تنفيذ هذه الخطوة تلقائيا. ومع ذلك، قد لا تكون النتائج مرضية دائما في نهاية المطاف. </li></ol></li><li style=";text-align:right;direction:rtl"> أداء العضلة الشادة حساب والخطوة الرابعة والأخيرة، <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> التأكد FA / DEC / ADC هم على. إن لم يكن انقر ON. </li><li style=";text-align:right;direction:rtl"> انقر على حساب. هذا الإجراء سوف يستغرق سوى بضع ثوان. </li></ol></li></ol></li><li style=";text-align:right;direction:rtl"> حفظ كافة البيانات والاستمرار مع fibertracking. لا تتوقف دون حفظ كل شيء. </li></ol> 3. Fibertracking ملاحظة: التشريحية معرفة مسار البصرية مهمة جدا للنتيجة ناجحة. <ol st</html style=";text-align:right;direction:rtl">مط = &quot;هامش اليسار: 40px؛&quot;&gt; <li style=";text-align:right;direction:rtl"> تحضير للعثور على ثلاث نقاط مهمة حيث تتمتع الألياف من خلال الذهاب. </li><li style=";text-align:right;direction:rtl"> تحديد chiasm البصرية باستخدام المعرفة التشريحية. <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> استخدام العائد على الاستثمار كنقطة انطلاق والسماح للألياف تمر. وتعرف رويس من قبل الطبيب. </li><li style=";text-align:right;direction:rtl"> بدلا من ذلك، جزء من المنطقة المشتبه بهم. انقر الإنقسام في أسفل اليسار وستظهر نافذة أخرى. وتعرف مناطق مجزأة تشريحيا المناطق. <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> رسم المنطقة يدويا. انتقل إلى أعلى وأسفل لتشمل chiasm البصرية بأكملها. حفظ الإجراء والعودة. </li></ol></li><li style=";text-align:right;direction:rtl"> تتبع الألياف سواء من المنطقة أو من الفائدة من منطقة مجزأة أو كليهما. </li><li style=";text-align:right;direction:rtl"> الألياف تصل إلى النواة الركبية اليسرى (LGN)، والتي هي النقطة الهامة الثانية للمسار البصري. كانت أقصى الزاوية 50. سوف خطر مساحات كاذبة ترتفع مع إذا كانت زاوية مرتفعة جدا. <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> هناك إمكانية لجزء من LGN كما هو موضح مع chiasm البصريةثم تتبع الألياف. بعد أن مجزأة chiasm البصرية، الألياف المسار التي تعمل من LGN والنهاية في chiasm البصرية أو العكس بالعكس. </li></ol></li><li style=";text-align:right;direction:rtl"> شريحة القشرة البصرية. المضي قدما في مثل حالة chiasm البصرية. وهذا قد يستغرق بعض الوقت كصورة 3DT1 مرجح يحتوي على 160 شرائح. </li><li style=";text-align:right;direction:rtl"> تتبع الألياف من القشرة البصرية إلى LGN. فمن الممكن أن تتبع لهم من LGN إلى القشرة البصرية كذلك. </li><li style=";text-align:right;direction:rtl"> إذا غزت القشرة البصرية من الورم أو ذمة ثم استخدام المنطقة ذات الاهتمام بدلا من منطقة مجزأة ومن ثم السماح الألياف تعمل في اتجاه LGN. ملاحظة: إذا تم مجزأة وذمة في بعض الأحيان قد تغزو القشرة البصرية ثم بعد ذلك يمكن أن القشرة البصرية لا تكون قادرة على مجزأة تماما لأن الكمبيوتر &#39;ر التمييز بينهما. الذي سيصدره لماذا كان من الضروري وضع العائد على الاستثمار. </li><li style=";text-align:right;direction:rtl"> أكرر كل شيء لنصف الكرة الأرضية الأخرى. </li><li style=";text-align:right;direction:rtl"> نبدأ مع أول نصف الكرة صحية. ملاحظة: ومن الممكن أن تبدأ مع الآخر أيضا، ولكن قد يكون من الأسهل تتبع ألياف نصف الكرة صحية أولا لتصبح الفكرة الأولى حول الوضع. فإنه ليس إلزاميا، بل هو المشورة فقط. </li></ol></li><li style=";text-align:right;direction:rtl"> قطاع الآفة الدماغية وذمة. المضي قدما على النحو المذكور أعلاه في 3.2.2. <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> تعيين اللون لكل منطقة مجزأة أو آفة من أجل التمييز بشكل أفضل. </li></ol></li><li style=";text-align:right;direction:rtl"> حفظ الإجراء بعد كل خطوة في حالة الأحداث غير المتوقعة أو في حالة الطوارئ. </li><li style=";text-align:right;direction:rtl"> تصدير البيانات بالكامل محليا. فمن الممكن تصديره إلى غرفة العمليات مباشرة ولكنه يسن &#39;ر الموصى بها. <ol style=";text-align:right;direction:rtl"><li style=";text-align:right;direction:rtl"> الملف الصحفي ومن ثم تصدير 3D-كائنات. تأكد من تصدير فقط امتحان التنقل. </li><li style=";text-align:right;direction:rtl"> دون &#39;ر تصدير امتحان الهجين. </li></ol></li><li style=";text-align:right;direction:rtl"> أدخل الجمجمة. اختيار المريض المناسب ثم اضغط Stealthmerge. اختيار الصور 3DT1 مرجح كما امتحان المرجعية. </li><li style=";text-align:right;direction:rtl"> خلق نموذج 3D وإدراج كل شيء. </li><li style=";text-align:right;direction:rtl"> استيراد ط البياناتن غرفة العمليات. </li></ol>

<ol>
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A., Krings, T. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Imaging+of+postthalamic+visual+fiber+tracts+by+anisotropic+diffusion+weighted+MRI+and+diffusion+tensor+imaging:+principles+and+applications.">Imaging of postthalamic visual fiber tracts by anisotropic diffusion weighted MRI and diffusion tensor imaging: principles and applications.</a> European Journal of Radiology. 49, 91-104 (2004).</li><li>Sherbondy, A. J., Dougherty, R. F., Napel, S., Wandell, B. A. <a target="_blank" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Search&doptcmdl=Citation&defaultField=Title+Word&term=Identifying+the+human+optic+radiation+using+diffusion+imaging+and+fiber.">Identifying the human optic radiation using diffusion imaging and fiber.</a> J. Vis. 8 (10), (2008).</li></ol>

The authors declare that they have no competing financial interests.

زارة التجارة والصناعة هي تقنية تمكن جراح الاعصاب لتصور مساحات المادة البيضاء في الجسم الحي 8. المسار البصري هي واحدة من هذه المناطق. على الرغم من أن هذا الأسلوب يوفر الأطباء مع إمكانيات جديدة بشأن علاج المرضى الذين يعانون من إصابات بليغة فيما المناطق من ال?...

يستخدم التصوير نشر الموترة (DTI) لتصوير WMT غير جراحية في الدماغ البشري 1. وقد تم استخدامه في العقد الماضي للحد من مخاطر الإضرار المناطق بليغة في الدماغ أثناء الجراحة 1. وقد أجريت زارة التجارة والصناعة في خمسين مريضا بين مارس 2012 ويناير 2014 لتصوير مسار البصرية. زارة التجارة والصناعة قد تحسن المحافظة على المناطق بليغة في الدماغ أثناء الجراحة من خلال توفير المعلومات الهامة حول الموقع التشريحي للمساحات المادة البيضاء. تم إدماجه في التخطيط الاستراتيجي لاستئصال آفات الدماغ معقدة 1. ومع ذلك، فإن تصوير المسار البصري لا يزال يشكل تحديا لأنه لا يوجد معيار لمعلمات زارة التجارة والصناعة، ووضع وحدات التخزين البذور وتفسير النتائج 12. وقد تم تنفيذ خوارزميات مختلفة حتى الآن 19-21. ركزت بعض المناهج على الطرق القطعية 19، 22-25. آخرون يستخدمون أساليب الاحتمالية، 26،27،29. في الآونة الأخيرة، وتقنيات استخدام Q-الكرة الحقول موتر والتصوير نشر الطيفية العالية والزاوي قرار إنتشار التصوير (HARDI) يتم استخدامها لتصوير مساحات المادة البيضاء وغيرها المسار البصري 1،13-15،18. ومع ذلك، فإن الوقت اللازم لHARDI بشكل ملحوظ أطول ب 45 دقيقة، والبرنامج هو غير متوفر تجاريا ويؤكد التطبيقات العلمية 18. يبدو أن فترة التدريس لHARDI أن تكون أطول من لDTI 18. بروتوكول المعروضة هي سهلة ممكنا، ويمكن استخدامها للروتين اليومي في عمليات جراحة الأعصاب من أجل تجنب الإصابة بالأمراض وتحسين نتائج ما بعد الجراحة. الوقت الإضافي لهذا البروتوكول هو أقل من 9 دقائق وهو أسرع بكثير من غيرها من البروتوكولات 1،9،12،16. الاعتراف بحقيقة أن العديد من خوارزميات متطورة وضعت مؤخرا يقيد الورقنفسها لاستخدام البرمجيات المتاحة تجاريا وادارة الاغذية والعقاقير المعتمدة. ومع ذلك فإنه إلزامي أن تأخذ في الاعتبار القيود المفروضة على هذه التقنية التي يتم المذكورة أعلاه.

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dti of the visual pathway - white matter tracts and cerebral lesions

<html> زارة التجارة والصناعة هو الاسلوب الذي يحدد مساحات المادة البيضاء (WMT) غير جراحية في المرضى الأصحاء وغير الأصحاء باستخدام قياسات نشرها. على غرار الممرات البصرية (VP)، WMT غير مرئية مع التصوير بالرنين المغناطيسي التقليدي أو داخل الجراحة مع المجهر. سوف DIT مساعدة جراحي الأعصاب لمنع تدمير نائب الرئيس أثناء إزالة الآفات المجاورة لهذا WMT. وقد أجرينا زارة التجارة والصناعة على خمسين المرضى قبل وبعد الجراحة بين مارس 2012 إلى يناير 2014. للتنقل استخدمنا تسلسل 3DT1 مرجح. بالإضافة إلى ذلك، أجرينا المرجحة T2 وزارة التجارة والصناعة، متواليات. كانت المعايير المستخدمة، مجال الرؤية: 200 × 200 ملم، وسمك شريحة: 2 مم، واكتساب مصفوفة: 96 × 96 ذات العوائد voxels الخواص ما يقرب من 2 × 2 × 2 مم. وقد أجريت المحوري MRI خارج باستخدام 32 الاتجاه التدرج واحدة B0 الصورة. كنا صدى مستو التصوير (EPI) والأصول التصوير بالتوازي مع عامل التسارع من 2 و ب القيمة من 800 ق / مم ². كانت المرة المسح أقل من 9 دقائق. الأنف والحنجرة &quot;&gt; وزارة التجارة والصناعة البيانات التي تم الحصول عليها تم معالجتها باستخدام FDA وافقت على برنامج نظام الملاحة الجراحي الذي يستخدم نهجا مباشرا الألياف تتبع المعروفة باسم الألياف مهمة المتابعة المستمرة من قبل (FACT). ويستند هذا على انتشار الخطوط الفاصلة بين المناطق ذات الاهتمام ( ROI) والتي تم تعريفها من قبل الطبيب. وأقصى زاوية من 50، الاتحاد الانجليزي يبدأ بقيمة 0.10 وقيمة ADC توقف من 0.20 مم ² / ق كانت المعايير المستخدمة لtractography. هناك بعض القيود لهذه التقنية. في إطار زمني محدود اكتساب يفرض المقايضات في جودة الصورة. نقطة أخرى مهمة لا ينبغي إهمالها هو التحول الدماغ أثناء الجراحة. أما بالنسبة للMRI الأخير داخل المنطوق قد تكون مفيدة. وعلاوة على ذلك من مخاطر مساحات إيجابية أو سلبية كاذبة كاذبة يحتاج إلى أن تؤخذ بعين الاعتبار والتي قد تؤثر سلبا على النتائج النهائية.

<html> هذا البروتوكول يمكن الطبيب من تصوير أجزاء كبيرة من نائب الرئيس على نحو كاف. ويمكن استخدامه مع كمية قليلة من الوقت من أجل منع الأضرار في المرضى الذين يعانون من آفات الدماغ القادمة إلى المناطق بليغة. وتبين ضوابط ما بعد الجراحة أيضا نتائج جيدة. يصور نائب الرئيس في <stron...

Watch this Scientific Journal Video about DTI of the Visual Pathway - White Matter Tracts and Cerebral Lesions at JoVE.com

DTI of the Visual Pathway - White Matter Tracts and Cerebral Lesions

تم إجراء التصوير نشر الموترة (DTI) في محاولة لتصوير أجزاء كبيرة من الممر البصري. وكان الهدف هو استخدام افقت ادارة الاغذية والعقاقير القياسية محطة العمل التجارية التي يمكن استخدامها للروتين اليومي في محاولة للحد من الأضرار بعد العملية الجراحية للمسار البصري في المرضى.

زارة التجارة والصناعة من المسار البصرية - وايت المسألة شيتاغونغ والشلل الآفات

DTI is a technique that identifies white matter tracts (WMT) non-invasively in healthy and non-healthy patients using diffusion measurements. Similar to ...

dti-of-the-visual-pathway-white-matter-tracts-and-cerebral-lesions

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13.9K Views.  Centre Hospitalier de Luxembourg.  تم إجراء التصوير نشر الموترة (DTI) في محاولة لتصوير أجزاء كبيرة من الممر البصري. وكان الهدف هو استخدام افقت ادارة الاغذية والعقاقير القياسية محطة العمل التجارية التي يمكن استخدامها للروتين اليومي في محاولة للحد من الأضرار بعد العملية الجرا?...

Video: DTI of the Visual Pathway - White Matter Tracts and Cerebral Lesions

The Application Of Permanent Middle Cerebral Artery Ligation in the Mouse

Focal cerebral ischemia is among the most common type of stroke seen in patients. Due to the clinical significance there has been a prolonged effort to develop suitable animal models to study the events that unfold during ischemic insult. These techniques include transient or permanent, focal or global ischemia models using many different animal models, with the most common being rodents.
The permanent MCA ligation method which is also referred as pMCAo in the literature is used extensively as a focal ischemia model in rodents 1-6. This method was originally described for rats by Tamura et al. in 1981 7. In this protocol a craniotomy was used to access the MCA and the proximal regions were occluded by electrocoagulation. The infarcts involve mostly cortical and sometimes striatal regions depending on the location of the occlusion. This technique is now well established and used in many laboratories 8-13. Early use of this technique led to the definition and description of &#x201C;infarct core&#x201D; and &#x201C;penumbra&#x201D; 14-16, and it is often used to evaluate potential neuroprotective compounds 10, 12, 13, 17. Although the initial studies were performed in rats, permanent MCA ligation has been used successfully in mice with slight modifications 18-20 .
This model yields reproducible infarcts and increased post-survival rates. Approximately 80% of the ischemic strokes in humans happen in the MCA area 21 and thus this is a very relevant model for stroke studies. Currently, there is a paucity of effective treatments available to stroke patients, and thus there is a need for good models to test potential pharmacological compounds and evaluate physiological outcomes. This method can also be used for studying intracellular hypoxia response mechanisms in vivo. 
Here, we present the MCA ligation surgery in a C57/BL6 mouse. We describe the pre-surgical preparation, MCA ligation surgery and 2,3,5 Triphenyltetrazolium chloride (TTC) staining for quantification of infarct volumes.

Middle cerebral artery (MCA) ligation is a technique to study focal cerebral ischemia in animal models. In this method, the middle cerebral artery is exposed by craniotomy and ligated by cauterization. This method gives highly reproducible infarct volumes and increased post-operative survival rates compared to other methods available.

تطبيق الربط الدائم الشريان المخي الأوسط في الماوس

Focal cerebral ischemia is among the most common type of stroke seen in patients. Due to the clinical significance there has been a prolonged effort to ...

Driving Simulation in the Clinic: Testing Visual Exploratory Behavior in Daily Life Activities in Patients with Visual Field Defects

Patients suffering from homonymous hemianopia after infarction of the posterior cerebral artery (PCA) report different degrees of constraint in daily life, despite similar visual deficits. We assume this could be due to variable development of compensatory strategies such as altered visual scanning behavior. Scanning compensatory therapy (SCT) is studied as part of the visual training after infarction next to vision restoration therapy. SCT consists of learning to make larger eye movements into the blind field enlarging the visual field of search, which has been proven to be the most useful strategy1, not only in natural search tasks but also in mastering daily life activities2. Nevertheless, in clinical routine it is difficult to identify individual levels and training effects of compensatory behavior, since it requires measurement of eye movements in a head unrestrained condition. Studies demonstrated that unrestrained head movements alter the visual exploratory behavior compared to a head-restrained laboratory condition3. Martin et al.4 and Hayhoe et al.5 showed that behavior demonstrated in a laboratory setting cannot be assigned easily to a natural condition. Hence, our goal was to develop a study set-up which uncovers different compensatory oculomotor strategies quickly in a realistic testing situation: Patients are tested in the clinical environment in a driving simulator. SILAB software (Wuerzburg Institute for Traffic Sciences GmbH (WIVW)) was used to program driving scenarios of varying complexity and recording the driver's performance. The software was combined with a head mounted infrared video pupil tracker, recording head- and eye-movements (EyeSeeCam, University of Munich Hospital, Clinical Neurosciences).
The positioning of the patient in the driving simulator and the positioning, adjustment and calibration of the camera is demonstrated. Typical performances of a patient with and without compensatory strategy and a healthy control are illustrated in this pilot study. Different oculomotor behaviors (frequency and amplitude of eye- and head-movements) are evaluated very quickly during the drive itself by dynamic overlay pictures indicating where the subjects gaze is located on the screen, and by analyzing the data. Compensatory gaze behavior in a patient leads to a driving performance comparable to a healthy control, while the performance of a patient without compensatory behavior is significantly worse. The data of eye- and head-movement-behavior as well as driving performance are discussed with respect to different oculomotor strategies and in a broader context with respect to possible training effects throughout the testing session and implications on rehabilitation potential.

Patients with visual deficits after stroke report about different constraints in daily life most likely due to variable compensatory strategies, which are difficult to differentiate in clinical routine. We present a clinical set-up which allows measurement of different compensatory head- and eye-movement-strategies and evaluating their effects on driving performance.

القيادة المحاكاة في عيادة: اختبار السلوك الاستكشافي البصرية في أنشطة الحياة اليومية في المرضى الذين يعانون من عيوب المجال البصري

Patients suffering from homonymous hemianopia after infarction of the posterior cerebral artery (PCA) report different degrees of constraint in daily ...

Optimized System for Cerebral Perfusion Monitoring in the Rat Stroke Model of Intraluminal Middle Cerebral Artery Occlusion

The translational potential of pre-clinical stroke research depends on the accuracy of experimental modeling. Cerebral perfusion monitoring in animal models of acute ischemic stroke allows to confirm successful arterial occlusion and exclude subarachnoid hemorrhage. Cerebral perfusion monitoring can also be used to study intracranial collateral circulation, which is emerging as a powerful determinant of stroke outcome and a possible therapeutic target. Despite a recognized role of Laser Doppler perfusion monitoring as part of the current guidelines for experimental cerebral ischemia, a number of technical difficulties exist that limit its widespread use. One of the major issues is obtaining a secure and prolonged attachment of a deep-penetration Laser Doppler probe to the animal skull. In this video, we show our optimized system for cerebral perfusion monitoring during transient middle cerebral artery occlusion by intraluminal filament in the rat. We developed in-house a simple method to obtain a custom made holder for twin-fibre (deep-penetration) Laser Doppler probes, which allow multi-site monitoring if needed. A continuous and prolonged monitoring of cerebral perfusion could easily be obtained over the intact skull.

Cerebral perfusion monitoring has been demonstrated to improve accuracy in ischemic stroke models. Technical difficulties often limit the use of this essential tool for cerebrovascular research. In this video, an optimized system is shown to obtain a single or multi-site hemodynamic monitoring during intraluminal middle cerebral artery occlusion in rats.

الأمثل نظام لرصد الإرواء الدماغي في نموذج الفئران من السكتة الدماغية داخل اللمعة انسداد الشريان الدماغي الأوسط

The translational potential of pre-clinical stroke research depends on the accuracy of experimental modeling. Cerebral perfusion monitoring in animal ...

Permanent Cerebral Vessel Occlusion via Double Ligature and Transection

Stroke is a leading cause of death, disability, and socioeconomic loss worldwide. The majority of all strokes result from an interruption in blood flow (ischemia) 1. Middle cerebral artery (MCA) delivers a great majority of blood to the lateral surface of the cortex 2, is the most common site of human stroke 3, and ischemia within its territory can result in extensive dysfunction or death 1,4,5. Survivors of ischemic stroke often suffer loss or disruption of motor capabilities, sensory deficits, and infarct. In an effort to capture these key characteristics of stroke, and thereby develop effective treatment, a great deal of emphasis is placed upon animal models of ischemia in MCA.
Here we present a method of permanently occluding a cortical surface blood vessel. We will present this method using an example of a relevant vessel occlusion that models the most common type, location, and outcome of human stroke, permanent middle cerebral artery occlusion (pMCAO). In this model, we surgically expose MCA in the adult rat and subsequently occlude via double ligature and transection of the vessel. This pMCAO blocks the proximal cortical branch of MCA, causing ischemia in all of MCA cortical territory, a large portion of the cortex. This method of occlusion can also be used to occlude more distal portions of cortical vessels in order to achieve more focal ischemia targeting a smaller region of cortex. The primary disadvantages of pMCAO are that the surgical procedure is somewhat invasive as a small craniotomy is required to access MCA, though this results in minimal tissue damage. The primary advantages of this model, however, are: the site of occlusion is well defined, the degree of blood flow reduction is consistent, functional and neurological impairment occurs rapidly, infarct size is consistent, and the high rate of survival allows for long-term chronic assessment.

Permanent Cerebral Vessel Occlusion via Double Ligature and Transection

We describe a highly reproducible method for the permanent occlusion of a rodent major cerebral blood vessel. This technique can be accomplished with very little peripheral damage, minimal blood loss, a high rate of long-term survival, and consistent infarct volume commensurate with the human clinical population.

الدائم انسداد الأوعية الدماغية<em&gt; عبر</em&gt; ضمد مزدوجة وعانى من قطع

Stroke is a leading cause of death, disability, and socioeconomic loss worldwide. The majority of all strokes result from an interruption in blood flow ...

Generation and 3-Dimensional Quantitation of Arterial Lesions in Mice Using Optical Projection Tomography

The generation and analysis of vascular lesions in appropriate animal models is a cornerstone of research into cardiovascular disease, generating important information on the pathogenesis of lesion formation and the action of novel therapies. Use of atherosclerosis-prone mice, surgical methods of lesion induction, and dietary modification has dramatically improved understanding of the mechanisms that contribute to disease development and the potential of new treatments.
Classically, analysis of lesions is performed ex vivo using 2-dimensional histological techniques. This article describes application of optical projection tomography (OPT) to 3-dimensional quantitation of arterial lesions. As this technique is non-destructive, it can be used as an adjunct to standard histological and immunohistochemical analyses.
Neointimal lesions were induced by wire-insertion or ligation of the mouse femoral artery whilst atherosclerotic lesions were generated by administration of an atherogenic diet to apoE-deficient mice.
Lesions were examined using OPT imaging of autofluorescent emission followed by complementary histological and immunohistochemical analysis. OPT clearly distinguished lesions from the underlying vascular wall. Lesion size was calculated in 2-dimensional sections using planimetry, enabling calculation of lesion volume and maximal cross-sectional area. Data generated using OPT were consistent with measurements obtained using histology, confirming the accuracy of the technique and its potential as a complement (rather than alternative) to traditional methods of analysis.
This work demonstrates the potential of OPT for imaging atherosclerotic and neointimal lesions. It provides a rapid, much needed ex vivo technique for the routine 3-dimensional quantification of vascular remodelling.

Ex vivo analysis of arterial lesions from animal models of cardiovascular disease classically relies on histological and immunohistochemical techniques. These provide 2-dimensional measurements in 3-dimensional lesions. This manuscript describes the generation of arterial lesions for quantitative analysis in 3-dimensions using optical projection tomography.

جيل والكميات 3-الأبعاد من الآفات الشرياني في الفئران عن طريق الإسقاط الضوئي المقطعي

The generation and analysis of vascular lesions in appropriate animal models is a cornerstone of research into cardiovascular disease, generating ...

Quantitative Analysis and Characterization of Atherosclerotic Lesions in the Murine Aortic Sinus

Atherosclerosis is a disease of the large arteries and a major underlying cause of myocardial infarction and stroke. Several different mouse models have been developed to facilitate the study of the molecular and cellular pathophysiology of this disease. In this manuscript we describe specific techniques for the quantification and characterization of atherosclerotic lesions in the murine aortic sinus and ascending aorta. The advantage of this procedure is that it provides an accurate measurement of the cross-sectional area and total volume of the lesion, which can be used to compare atherosclerotic progression across different treatment groups. This is possible through the use of the valve leaflets as an anatomical landmark, together with careful adjustment of the sectioning angle. We also describe basic staining methods that can be used to begin to characterize atherosclerotic progression. These can be further modified to investigate antigens of specific interest to the researcher. The described techniques are generally applicable to a wide variety of existing and newly created dietary and genetically-induced models of atherogenesis.

We describe procedures to quantify and characterize atherosclerotic lesions in mouse models using precision sectioning of the aortic sinus and ascending aorta combined with histochemical and immunohistochemical analysis.

Atherosclerosis is a disease of the large arteries and a major underlying cause of myocardial infarction and stroke. Several different mouse models have ...

Dynamic Visual Tests to Identify and Quantify Visual Damage and Repair Following Demyelination in Optic Neuritis Patients

In order to follow optic neuritis patients and evaluate the effectiveness of their treatment, a handy, accurate and quantifiable tool is required to assess changes in myelination at the central nervous system (CNS). However, standard measurements, including routine visual tests and MRI scans, are not sensitive enough for this purpose. We present two visual tests addressing dynamic monocular and binocular functions which may closely associate with the extent of myelination along visual pathways. These include Object From Motion (OFM) extraction and Time-constrained stereo protocols. In the OFM test, an array of dots compose an object, by moving the dots within the image rightward while moving the dots outside the image leftward or vice versa. The dot pattern generates a camouflaged object that cannot be detected when the dots are stationary or moving as a whole. Importantly, object recognition is critically dependent on motion perception. In the Time-constrained Stereo protocol, spatially disparate images are presented for a limited length of time, challenging binocular 3-dimensional integration in time. Both tests are appropriate for clinical usage and provide a simple, yet powerful, way to identify and quantify processes of demyelination and remyelination along visual pathways. These protocols may be efficient to diagnose and follow optic neuritis and multiple sclerosis patients.
In the diagnostic process, these protocols may reveal visual deficits that cannot be identified via current standard visual measurements. Moreover, these protocols sensitively identify the basis of the currently unexplained continued visual complaints of patients following recovery of visual acuity. In the longitudinal follow up course, the protocols can be used as a sensitive marker of demyelinating and remyelinating processes along time. These protocols may therefore be used to evaluate the efficacy of current and evolving therapeutic strategies, targeting myelination of the CNS.

Object From Motion (OFM) and Time-constrained stereo protocols are sensitive tools to identify monocular and binocular dynamic visual function deficits, which are uniquely affected in optic neuritis patients. Furthermore, these tests may be used as quantitative noninvasive tools to assess the extent of myelination along visual pathways.

الاختبارات البصرية الديناميكية لتحديد وقياس الأضرار البصرية والتصليح وبعد إزالة الميالين في التهاب العصب البصري المرضى

In order to follow optic neuritis patients and evaluate the effectiveness of their treatment, a handy, accurate and quantifiable tool is required to ...

A Versatile Murine Model of Subcortical White Matter Stroke for the Study of Axonal Degeneration and White Matter Neurobiology

Stroke affecting white matter accounts for up to 25% of clinical stroke presentations, occurs silently at rates that may be 5-10 fold greater, and contributes significantly to the development of vascular dementia. Few models of focal white matter stroke exist and this lack of appropriate models has hampered understanding of the neurobiologic mechanisms involved in injury response and repair after this type of stroke. The main limitation of other subcortical stroke models is that they do not focally restrict the infarct to the white matter or have primarily been validated in non-murine species. This limits the ability to apply the wide variety of murine research tools to study the neurobiology of white matter stroke. Here we present a methodology for the reliable production of a focal stroke in murine white matter using a local injection of an irreversible eNOS inhibitor. We also present several variations on the general protocol including two unique stereotactic variations, retrograde neuronal tracing, as well as fresh tissue labeling and dissection that greatly expand the potential applications of this technique. These variations allow for multiple approaches to analyze the neurobiologic effects of this common and understudied form of stroke.

Here we present methodology for the production of a focal stroke in murine white matter by local injection of an irreversible endothelial nitric oxide synthase (eNOS) inhibitor (L-Nio). Presented are two stereotactic variations, retrograde neuronal tracing, and fresh tissue labeling and dissection that expand the potential applications of this technique.

والسكتة الدماغية تنوعا فأري موديل تحت القشرية الأبيض المسألة لدراسة محور عصبي تنكس والأبيض المواد بيولوجيا الأعصاب

Stroke affecting white matter accounts for up to 25% of clinical stroke presentations, occurs silently at rates that may be 5-10 fold greater, and ...

Autologous Microfractured and Purified Adipose Tissue for Arthroscopic Management of Osteochondral Lesions of the Talus

In recent years, regenerative techniques have been increasingly studied and used to treat osteochondral lesions of the talus. In particular, several studies have focused their attention on mesenchymal stem cells derived from adipose tissue. Adipose-derived stem cells (ADSCs) exhibit morphological characteristics and properties similar to other mesenchymal cells, and are able to differentiate into several cellular lines. Moreover, these cells are also widely available in the subcutaneous tissue, representing 10 - 30% of the normal body weight, with a concentration of 5,000 cells per gram of tissue.
In the presented technique, the first step involves harvesting ADSCs from the abdomen and a process of microfracture and purification; next, the surgical procedure is performed entirely arthroscopically, with less soft tissue dissection, better joint visualization, and a faster recovery compared with standard open procedures. Arthroscopy is characterized by a first phase in which the lesion is identified, isolated, and prepared with microperforations; the second step, performed dry, involves injection of adipose tissue at the level of the lesion.
Between January 2016 and September 2016, four patients underwent arthroscopic treatment of osteochondral lesion of the talus with microfractured and purified adipose tissue. All patients reported clinical improvement six months after surgery with no reported complications. Functional scores at the latest follow-up are encouraging and confirm that the technique provides reliable pain relief and improvements in patients with osteochondral lesion of the talus.

The aim of this study is to report a protocol for the arthroscopic treatment of osteochondral lesions of the talus using microfractured and purified adipose-derived stem cells.

ميكروفراكتوريد ذاتي وتنقية الأنسجة الدهنية لإدارة الآفات Osteochondral لميلان المنظار

In recent years, regenerative techniques have been increasingly studied and used to treat osteochondral lesions of the talus. In particular, several ...

Assessment of Static Graviceptive Perception in the Roll-Plane using the Subjective Visual Vertical Paradigm

Vestibular disorders are among the most common syndromes in medicine. In recent years, new vestibular diagnostic systems have been introduced that allow the examination of all semicircular canals in the clinical setting. Assessment methods of the otolithic system, which is responsible for the perception of linear acceleration and perception of gravity, are far less in clinical use. There are several experimental approaches for measuring the perception of gravity. The most frequently used method is the determination of the subjective visual vertical. This is usually measured with the head in an upright position. We present here an assessment method for testing otolith function in the roll plane. The subjective visual vertical is measured in the head upright position as well as with head inclination of &#177; 15&#176; and &#177; 30&#176; in the roll plane. This extended functional paradigm is an easy-to-perform clinical test of otolith function and ensures increased information content for the detection of impaired graviceptive perception.

The perception of gravity is commonly determined by the subjective visual vertical in the head upright position. The additional assessment at head tilts of &#177; 15&#176; and &#177; 30&#176; in the roll plane ensures increased information content for the detection of impaired graviceptive perception.

تقييم التصور اللاستاتيكي في المستوى الأعلى باستخدام النموذج العمودي البصري الذاتي

Vestibular disorders are among the most common syndromes in medicine. In recent years, new vestibular diagnostic systems have been introduced that allow ...