A Standardized Approach for Multispecies Purification of Mammalian Male Germ Cells by Mechanical Tissue Dissociation and Flow Cytometry

11.6K Views

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08:46 min

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July 12th, 2017

DOI :

10.3791/55913-v

July 12th, 2017

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Ana C. Lima*¹^,²^,³^,⁴, Min Jung*¹, Jannette Rusch¹, Abul Usmani¹, Alexandra M. Lopes³^,⁴, Donald F. Conrad¹

¹Department of Genetics, Washington University School of Medicine, ²Graduate Program in Areas of Basic and Applied Biology (GABBA), Abel Salazar Institute of Biomedical Sciences, University of Porto, ³Instituto de Investigação e Inovação em Saúde, University of Porto, ⁴IPATIMUP - Instituto de Patologia e Imunologia Molecular da Universidade do Porto, ⁵Department of Pathology & Immunology, Washington University School of Medicine

Summary

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Keywords Mammalian Male Germ Cells

Fluorescence activated Cell Sorting

Standardized Protocol

Multispecies

Tissue Disaggregation

Cell Suspension

Cell Filtration

Chapters in this video

0:05

Title

1:10

Testicular Tissue Single Cell Suspension Generation

2:26

Cell Staining and Fluorescence Activiated Cell Sorting (FACS)

5:33

Results: Representative Testicular Cell Subset Analyses

7:17

Conclusion

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