Differentiation of Human Pluripotent Stem Cells Into Pancreatic Beta-Cell Precursors in a 2D Culture System

2.5K Views

10:12 min

December 16th, 2021

DOI :

10.3791/63298-v

December 16th, 2021


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Human Pluripotent Stem Cells

Chapters in this video

0:05

Introduction

1:22

Induction of Definitive Endoderm Differentiation in Human Pluripotent Stem Cells (hPSCs) (Stage 1)

2:10

Immunofluorescence Analysis of hPSC-Derived Definitive Endoderm (Stage 1)

4:19

Generation of the Primitive Gut Tube from hPSCs (Stage 2)

5:50

Assessment of Differentiation Efficiency of Generating Pancreatic Progenitors

8:10

Results: Comparison between Optimized and Non-Optimized Protocol for Scalable Generation of Pancreatic Progenitors from hPSCs

9:21

Conclusion

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