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In Vitro Pancreas Organogenesis from Dispersed Mouse Embryonic Progenitors

DOI :

10.3791/51725-v

July 19th, 2014

July 19th, 2014

13,552 Views

1Ecole Polytechnique Fédérale de Lausanne, School of Life Sciences, Swiss Institute for Experimental Cancer Research, 2DanStem, University of Copenhagen

The three-dimensional culture method described in this protocol recapitulates pancreas development from dispersed embryonic mouse pancreas progenitors, including their substantial expansion, differentiation and morphogenesis into a branched organ. This method is amenable to imaging, functional interference and manipulation of the niche.

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In Vitro Pancreas Organogenesis

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