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Neuroscience

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Methods Article

Free-Hand Intracerebroventricular Injections in Mice

9.5K Views

•

05:29 min

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January 12th, 2024

DOI :

10.3791/65324-v

January 12th, 2024

•

Richard B. McCosh¹, Lauren A. Young¹

¹Department of Biomedical Sciences, Colorado State University

Transcript

We are interested in the neural control of reproductive function, particularly how the brain regulates the release of hormones that control the gonads, both in healthy conditions and in response to stress. The main advantage of this technique is that it is relatively simple and rapid. Delivering drugs into the lateral ventricle is a great way to cast a wide net in testing a signaling pathway to support more detailed follow-up experiments.

There's still much to be learned about the neuroendocrine control of reproduction, including the molecular mechanisms for luteinizing hormone pulse generation, mechanisms for modulating pulse frequency during stress, and generation of the preovulatory LH surge. In the future, we are particularly interested in integrating disparate findings into models.

Summary

Here, a simple and rapid approach for performing intracerebroventricular injections in mice using a free-hand approach (that is, without a stereotaxic device) is described.

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Free hand Intracerebroventricular Injections

Reproductive Function

Hormone Release

Lateral Ventricle

Signaling Pathway

Neuroendocrine Control

Luteinizing Hormone

Stress Response

Molecular Mechanisms

Experimental Agents

Glass Syringe Method

Isoflurane Anesthesia

In Vivo Recording

Chapters in this video

0:00

Introduction

0:57

Mouse Craniotomy for Free‐Hand Intracerebroventricular Injection

3:07

Intracerebroventricular Injection in Mice

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