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Injections of AAV Vectors for Optogenetics in Anesthetized and Awake Behaving Non-Human Primate Brain (Video) | JoVE

DOI :

10.3791/62546-v

9:42 min

August 4th, 2021

August 4th, 2021

2,958 Views

1Dept. of Otolaryngology - Head and Neck Surgery, University of Washington, 2Washington National Primate Research Center, University of Washington, 3Allen Institute for Brain Science, 4Dept. of Physiology & Biophysics, University of Washington

As currently implemented, optogenetics in non-human primates requires injection of viral vectors into the brain. An optimal injection method should be reliable and, for many applications, capable of targeting individual sites of arbitrary depth that are readily and unambiguously identified in postmortem histology. An injection method with these properties is presented.

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