Preparation of Legionella pneumophila Cultures for Priming Stage Infections
4:14
Priming Stage Infection Using Acanthamoeba castellanii
7:13
Processing of Bacteria for Target Cell Stage Infection after the Priming Stage Infection
9:46
Experimental Analysis
11:14
CFU Plating
11:51
Representative Infection Results
13:23
Conclusion
This technique provides a method to harvest, normalize and quantify intracellular growth of bacterial pathogens that are pre-cultivated in natural protozoan host cells prior to infections of mammalian cells. This method can be modified to accommodate a wide variety of host cells for the priming stage as well as target cell types.
