用于可视化神经突在冷冻水合状态下使用低温电子断层扫描准备初级神经元
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09:59 min
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February 12th, 2014
DOI :
February 12th, 2014
•Sarah H. Shahmoradian1, Mauricio R. Galiano2, Chengbiao Wu3, Shurui Chen4, Matthew N. Rasband2, William C. Mobley3, Wah Chiu4
1Department of Molecular Physiology and Biophysics, Baylor College of Medicine, 2Department of Neuroscience, Baylor College of Medicine, 3Department of Neuroscience, University of California at San Diego, 4National Center for Macromolecular Imaging, Verna and Marrs McLean Department of Biochemistry and Molecular Biology, Baylor College of Medicine
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此视频中的章节
0:05
Title
1:19
Preparing Dishes with EM Grids
3:43
Preparing and Plating Primary Neurons on EM Grids
4:45
Vitrifying Neurons on EM Grids
8:05
Results: Visualizing Neurites in a Frozen-hydrated State Using Cryo-Electron Tomography
9:32
Conclusion
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