The authors would like to acknowledge the Cincinnati Children&#8217;s Hospital Division of Veterinary Services for their assistance. This manuscript was supported by the National Institutes of Health K12 HD028827. Anna Perl PhD taught the authors this surgical procedure.

注：动物使用声明：在本研究中的所有程序均经批准，并按照机构动物使用及护理委员会（IACUC）在辛辛那提儿童医院的指导方针进行。八周龄C57BL / 6J雄性小鼠从Jackson实验室（巴港，ME）中获得，并使其适应环境，使用前一个星期。直到手术，将动物饲养在无病原体的阻挡设施，并提供高压灭菌食物和过滤水的广告libdium。每个鼠笼用专用的空气和水的供给，并提供维持在12小时昼夜周期。以下从手术中恢复，小鼠保持在笼子里与过滤上衣，提供蒸压州城广告libidum，并提供了从一瓶水过滤水。 1.准备仪器<ol><li>使用曲别针和大头针6皮肤拉钩。扭曲拉直回形针在纸张的针柄，升屋檐5cm的直钢丝的一端，使单个0.5厘米“U”形钩在导线的末端。 </li><li>使用保鲜膜约15×15厘米见方的手术单。准备每只小鼠1敷料。把一纸塔在每个包装之间。 </li><li>消毒所有的手术工具以及一叠12×12英寸的软木砖，纱布，棉花拭子。 </li></ol> 2.鼠标准备<ol><li>诱导麻醉，用2％异氟醚。称量动物。 </li><li>在一个专门的手术准备区刮胡子左胸部和颈部电气剃须刀。 </li><li>申请一滴人工泪液软膏鼠标的眼睛。 </li><li>净化颈部和左胸部与洗必泰和异丙醇。重复两次以上。 </li></ol> 3.鼠标奥罗气管插管和机械通气<ol><li>有一个非无菌手术技术人员代替鼠标仰卧在预热手术区。</li><li>通过记录缺乏爪子掐的响应确认麻醉深度。 </li><li>洗手，并穿上手术装束，口罩，帽子后，穿上无菌手术手套。 </li><li>立体裁剪，并使用无菌技术后，使超过前中期的脖子，露出喉部有1厘米的垂直切口。轻轻缩回带状肌与弯曲的，锯齿10厘米钳，并通过与一个直剪刀的前端扩展的带状肌暴露喉和气管。 </li><li>口服插入一个22克钝尖血管导管插入中期气管（ 图1A）和目视确认放置（ 图1B）。维持麻醉和通风用1-3％异氟醚通过啮齿动物呼吸机（225微升每冲程;每分钟200斯托克斯）。采用15厘米H 2 O的压力极限</li></ol> 4.鼠标肺切除<ol><li>莱鼠标在右侧卧位，用鼠标的背面朝上操作员（左侧上）。用自密封的保鲜膜作为无菌的悬垂性。切割通过悬垂性好，用钝尖剪子弯，使长切割平行于肋骨2厘米，在第 4和第 5肋间。将钝头剪刀弯曲和底层的肋骨和肋间肌肉解剖皮肤了。 </li><li>缩回四个拉钩皮肤暴露正方形1.5×1.5cm的外科窗口（ 图2A）。固定拉钩，以软木板上。 </li><li>解剖下来使用弯钳肋，并使用弯钳之一尖端进入胸腔。 </li><li>使用钝头微型剪刀，使用下刀片进入胸腔。使肋和重复在相反的方向之间为0.5厘米的切口。 </li><li>使用剩下的两个拉钩，在前后轴和安全的拉钩到软木板上（ 图2B）打开胸腔。 </li> &lt;李&gt;使用在左手弯曲钝尖镊子，把握左肺和置换左肺的上部横向和下方通过开胸直到左肺动脉与支气管露出（ 图3A，B）。 </li><li>持在右手装入钛血管microclip敷贴与涂抹在手掌和弯曲尖端从手掌（ 图3C）背向的主体中，沿左侧的后方面的曲率滑动施用尖端进入胸腔肺和剪辑左支气管和肺动脉（ 图3D）。 </li><li>取下敷贴但保留左肺缩回。抓住钝尖微型剪刀用右手切支气管和肺动脉远端剪辑，删除左肺（ 图3E）。 </li><li>拆下肋骨拉钩。 </li><li>使用弯曲钝钳捏起来1厘米皮肤逊色于incisi上，但上述隔膜的水平，并插入穿过皮肤并进入左胸腔（ 图4A，B）的一个24克血管导管。 </li><li>使用5-0 prolene线缝合周围放置第 4 次 2间断缝合和第 5肋关闭胸腔。 </li><li>去除皮肤拉钩。使用两套镊子来近似沿切口的长度皮肤和粘在皮肤闭合。 </li><li>一个3毫升路厄锁注射器连接到血管导管和通过施加轻柔抽吸抽出血管导管除去残留的空气。 </li><li>胶颈部切口闭合使用镊子两套像以前一样。 </li></ol> 5.鼠标深水肺切除<ol><li>暴露左肺如“老鼠肺切除”协议指出。提起肋笼具有弯曲钝钳，以允许空气进入左胸腔（ 图5A，B）。 </li><li>放置一个24克血管导管插入左寿拉契奇腔上面，小心不要弄伤左肺。 </li><li>使用5-0普理灵缝合小心不要刺破肺（ 图5C），将两个长度的缝合材料到3 次 / 4和第 5/6 次肋骨间隙（ 图5D）。绑减轻左肺症的风险之前，将缝合材料的两个长度。领带的缝合材料，以使2中断线圈（ 图5E）。 </li><li>胶上的皮肤胸切开，除去残留的空气与血管导管，和胶水颈部切口如上。 </li></ol> 6.复苏，镇痛和恢复<ol><li>关掉异氟烷，并管理0.1毫克/公斤丁丙诺啡和0.5生理盐水皮下注射毫升。 </li><li>当自主呼吸恢复，取出气管插管。 </li><li>观察鼠标，直到它再次走动。通常走resum上课除去气管导管后几分钟。 </li><li>放置在27℃培养箱（加湿，25％氧气）鼠标恢复O / N。 注意：我们放置食物与水润湿的上箱地板为手术后的第一个24小时的几个粒料。 </li><li>手术后每日三天两次施用0.1毫克/千克丁丙诺啡腹腔注射。小心不要处理的动物时，打开手术部位。 </li></ol> 7.鼠标监测<ol><li>手术后称量小鼠在1，3,5和7天。 </li></ol>

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The authors have nothing to disclose.

我们已经提供了迄今报告的鼠标PNX和鼠标假PNX程序的最详细的描述。我们已经取得的读者了解一些常见的陷阱，调查人员学习的过程通常会遇到，我们已经介绍了我们的实验室开发，以减轻对这些缺陷的几个技巧。利用这种模式其他实验室可能已经开发了其他的技术修改或使用不同的仪器。在评估的技术差异，个别研究者将需要决定是否这些差异显著影响实验结果。 补偿性?...

肺的主要功能是提供一个有机体和大气之间的氧气和二氧化碳交换。在人类中，一台主机的先天和后天条件导致降低的肺表面面积导致肺功能受损。虽然宿主疗法，如吸入皮质类固醇，支气管扩张剂，补充氧气和慢性机械通气用于减轻肺功能受损1-3的后果，提供了理想的治疗，这些条件将促进功能的肺组织的再生- 即，肺再生。 哺乳动物组织再生已经有据可查。非洲刺鼠可再生大面积皮肤无疤痕形成4。在人类的末节指骨可再生损伤后或截肢5-7。继肺（PNX），肺代偿出现增长小鼠8只9，做GS 10和人类11。根据定义，补偿性肺生长包括现有的空域与扩展相关的微循环12不仅扩大这些扩大空域，但重新septation。基因表达分析表明，这种模式概括很多肺发育13的信号事件。四周后小鼠PNX，肺泡表面面积相当于假手术的动物14。在这个手稿中，我们描述鼠标PNX和假PNX程序。

<table border="0" cellpadding="0" cellspacing="0" width="1052">
	<tbody>
		<tr height="22">
			<td height="22" style="height:22px;width:263px;">Name of the reagent</td>
			<td style="width:263px;">Company</td>
			<td style="width:263px;">Catalogue number</td>
			<td style="width:263px;">Comments (optional)</td>
		</tr>
		<tr height="101">
			<td height="101" style="height:101px;width:263px;">6-inch Vascular Clip Applicator</td>
			<td style="width:263px;">Teleflex Medical (WECK)</td>
			<td style="width:263px;">137062</td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="101">
			<td height="101" style="height:101px;width:263px;">Horizon Small Titanium Red Clip</td>
			<td style="width:263px;">Teleflex Medical (WECK)</td>
			<td style="width:263px;">1201</td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="121">
			<td height="121" style="height:121px;width:263px;">Narrow Pattern 12cm Curved Forceps</td>
			<td style="width:263px;">Fine Science Tools</td>
			<td style="width:263px;">11003-12</td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="141">
			<td height="141" style="height:141px;width:263px;">Curved Serated 10 cm Graefe Forceps&#160;</td>
			<td style="width:263px;">Fine Science Tools</td>
			<td style="width:263px;">11052-10</td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:263px;">Castroviejo Needle Holder</td>
			<td style="width:263px;">Fine Science Tools</td>
			<td style="width:263px;">12565-14</td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:263px;">Straight 9 cm Strabismus Scissors (Blunt Tip)</td>
			<td style="width:263px;">Fine Science Tools</td>
			<td style="width:263px;">14075-09</td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:263px;">Straight 8.5 cm Hardened Fine Scissors</td>
			<td style="width:263px;">Fine Science Tools</td>
			<td style="width:263px;">14090-09</td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:263px;">Straight, Blunt Tip Cohan-Vannas Spring Scissors</td>
			<td style="width:263px;">Fine Science Tools</td>
			<td style="width:263px;">15000-12</td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:263px;">Skin Glue</td>
			<td style="width:263px;">Gluture</td>
			<td style="width:263px;">32046</td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:263px;">22 ga angiocatheter</td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:263px;">24 ga angiocatheter</td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:263px;">3 mL luer lock syringe</td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:263px;">4 short retractors</td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:263px;">2 long retractors</td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="41">
			<td height="41" style="height:41px;width:263px;">5-0 Prolene on curved cutting needle</td>
			<td style="width:263px;">Ethicon</td>
			<td style="width:263px;">8698G</td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:263px;">0.5 mL syringe on 27 ga needle</td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:263px;">Normal Saline</td>
			<td style="width:263px;"></td>
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			<td style="width:263px;"></td>
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		<tr height="21">
			<td height="21" style="height:21px;width:263px;">Buprenorphine</td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:263px;">Press-n-Seal Wrap</td>
			<td style="width:263px;">Glad Products Company</td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:263px;">12 inch X 12 inch cork board stack</td>
			<td style="width:263px;">Office Depot</td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:263px;">70% Ethanol</td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:263px;">Betadine</td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:263px;">Mouse Ventilator</td>
			<td style="width:263px;">Hugo Sachs Elektronnik&#160;</td>
			<td style="width:263px;">Minivent Type 845</td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:263px;">Isoflurane Vaporizer</td>
			<td style="width:263px;">OHMEDA</td>
			<td style="width:263px;">Excel 210 SE</td>
			<td style="width:263px;"></td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:263px;">artificial tear ointment</td>
			<td style="width:263px;">puralube</td>
			<td style="width:263px;">NDC: 17033-211-38</td>
			<td style="width:263px;"></td>
		</tr>
	</tbody>
</table>

mouse pneumonectomy model of compensatory lung growth

In humans, disrupted repair and remodeling of injured lung contributes to a host of acute and chronic lung disorders which may ultimately lead to disability or death. Injury-based animal models of lung repair and regeneration are limited by injury-specific responses making it difficult to differentiate changes related to the injury response and injury resolution from changes related to lung repair and lung regeneration. However, use of animal models to identify these repair and regeneration signaling pathways is critical to the development of new therapies aimed at improving pulmonary function following lung injury. The mouse pneumonectomy model utilizes compensatory lung growth to isolate those repair and regeneration signals in order to more clearly define mechanisms of alveolar re-septation. Here, we describe our technique for performing mouse pneumonectomy and sham pneumonectomy. This technique may be utilized in conjunction with lineage tracing or other transgenic mouse models to define molecular and cellular mechanism of lung repair and regeneration.

。PNX，假手术鼠标重量的情节在图6提供了在我们的手中，生存始终是95 - 100％为PNX和假肺。有关如何右肺再生长在该模型和预期的时间过程中，我们建议读者参考吉布尼等人的 15和Wang 等人的手稿的描述。14 几种常见的陷阱，必须避免成功执行鼠标PNX和鼠标假肺的程序。 气管插管 ：许多研究者学?...

Watch this Scientific Journal Video about Mouse Pneumonectomy Model of Compensatory Lung Growth at JoVE.com

Mouse Pneumonectomy Model of Compensatory Lung Growth

Mouse pneumonectomy is a commonly employed model of compensatory lung growth. This procedure can be used in conjunction with lineage tracing or transgenic mouse models to elucidate underlying mechanisms.

补偿性增长龙鼠标肺切除型号

In humans, disrupted repair and remodeling of injured lung contributes to a host of acute and chronic lung disorders which may ultimately lead to ...

mouse-pneumonectomy-model-of-compensatory-lung-growth

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12.6K 次观看.  Cincinnati Children's Hospital Medical Center. Mouse pneumonectomy is a commonly employed model of compensatory lung growth. This procedure can be used in conjunction with lineage tracing or transgenic mouse models to elucidate underlying mechanisms.