Large Scale Antibody Production from Batch Overgrowth Culture
5:12
Antibody Purification by Affinity Chromatograpy Using an Automated Fast Protein Liquid Chromatography System
7:02
Results: Laboratory-scale Production and Purification of Antibody
8:50
Conclusion
Transcript
The overall goal of this method is to develop a high-yielding serum free expression system for the lab scale production of a therapeutic antibody. This method can address key considerations in the design of an antibody expression system such as pr
Sign in or start your free trial to access this content
This protocol describes the production of a therapeutic antibody in a mammalian expression system. The methods described include preparation of vector DNA, stable transfection and serum-free adaptation of a human embryonic kidney 293 cell line, set up of large scale cultures and purification using affinity chromatography.
