February 15th, 2019
•Here we present a direct intrathecal injection technique using 1% lidocaine hydrochloride in a viral solution to ensure efficient adeno-associated virus delivery to small animals and establish a scoring system to predict transduction efficiency in the central nervous system according to the degree of transient weakness induced by lidocaine.
Related Videos
Intracranial Injection of Adeno-associated Viral Vectors
Subpial Adeno-associated Virus 9 (AAV9) Vector Delivery in Adult Mice
Adeno-associated Virus-mediated Transgene Expression in Genetically Defined Neurons of the Spinal Cord
Transcranial Direct Current Stimulation (tDCS) in Mice
Production, Purification, and Quality Control for Adeno-associated Virus-based Vectors
Double Direct Injection of Blood into the Cisterna Magna as a Model of Subarachnoid Hemorrhage
Electrocorticographic Recording of Cerebral Cortex Areas Manipulated Using an Adeno-Associated Virus Targeting Cofilin in Mice
Author Spotlight: Automated Infusion and Blood Sampling for Precise Hormonal Analysis in Conscious Mice
Intracerebroventricular Injection in Mice (Video) | JoVE
Author Spotlight: Intrathecal Injection – An Efficient and Reliable Delivery Method to Test the Efficacy of Gene Editing in Neonatal Mouse Brains