Mouse Preparation and Direct Intrathecal Adeno-associated Virus (AAV) Delivery
3:16
Immunohistochemical Tissue Preparation
4:46
Immunohistochemistry
6:27
Results: Representative AAV Transduction After Direct Intrathecal Delivery
7:52
Conclusion
필기록
This method facilitates the efficient intrathecal delivery of adeno-associated viral transgenes in awake small animals for the experimental treatment of central nervous system diseases such as ALS. The main advantage of this method is that the vir
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Here we present a direct intrathecal injection technique using 1% lidocaine hydrochloride in a viral solution to ensure efficient adeno-associated virus delivery to small animals and establish a scoring system to predict transduction efficiency in the central nervous system according to the degree of transient weakness induced by lidocaine.