Results: Cardiac Chamber Development During Mouse Embryogenesis in MLC-2v-tdTomato Knock-in Mice
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Conclusion
副本
Using this method we can directly exam heart tube formation, looping, and full chamber formation without further experimental manipulations of the mouse embryo. Although, we use MLC-2v-tdTomato mice as an example, this simple method can be widely
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We present the protocols to examine mouse heart development using whole mount epifluorescent microscopy on mouse embryos dissected from ventricular specific MLC-2v-tdTomato reporter knock-in mice. This method allows us to directly visualize each stage of the ventricular formation during mouse heart development without labor-intensive histochemical methods.