Preparing Protein Lysates for Western Blotting from 3D Spheroid Cultures
5:21
Propidium Iodide (PI) Staining of Spheroids
6:27
Embedding of Spheroids
7:52
Results: Assessing Cell Viability and Death
9:24
Conclusion
Transcript
The protocols presented here have been optimized for 3D culture and allow for efficient and affordable investigation of processes associated with cancer cell growth, proliferation, and death in a 3D environment. The main advantages of using 3D cul
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Here, we present several simple methods for evaluating viability and death in 3D cancer cell spheroids, which mimic the physico-chemical gradients of in vivo tumors much better than the 2D culture. The spheroid model, therefore, allows evaluation of the cancer drug efficacy with improved translation to in vivo conditions.
