Design and Fabrication of a 3D Bifurcation of the Human Carotid Artery Model
2:18
Human Umbilical Vein Endothelial Cell (HUVEC) Seeding
3:26
Closed-Circuit Configuration: Perfusion Experiment and Imaging
4:42
Open-Circuit Configuration: Model Washing, Data Acquisition, and Analysis
5:36
Results: Microscopic Analysis of 3D Carotid Artery Model-Seeded Endothelial Cells
6:17
Conclusion
Transcript
The present protocol may serve as a new platform for the study and development of drug carriers to target disease sites within the human vascular system. The main advantage of our technique is that it allows the study of drug carriers'accumulation
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Here, we present a new protocol to study and map the targeted deposition of drug carriers to endothelial cells in fabricated, real-sized, three-dimensional human artery models under physiological flow. The presented method may serve as a new platform for targeting drug carriers within the vascular system.
