Results: The Effect of Sucrose Concentration on the Particle Size of Glycogen
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Conclusion
副本
This protocol allows for the extraction of the liver glycogen molecules in a manner that preserves their structure and limits the amount of the small glycogen particles lost. With previous research showing diabetes alter glycogen structure, this m
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An optimal sucrose concentration was determined for the extraction of liver glycogen using sucrose density gradient centrifugation. The addition of a 10 min boiling step to inhibit glycogen-degrading enzymes proved beneficial.