Imaging and Measuring Cell Shape, Size, and Microridge Pattern
6:36
Results: Imaging Analysis Using Scanning Electron Microscopy
7:28
Conclusion
副本
Corneal transparency is instrumental to clear sight. In pathological context, this transparency can be challenge. Studying pathogenesis mechanism requires simple models having a high similarity to human organ.
In these aspects, zebrafish is
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This protocol focuses on damaging the ocular surface of zebrafish through abrasion to assess the subsequent wound closure at the cellular level. This approach exploits an ocular burr to partly remove the corneal epithelium and uses scanning electron microscopy to track changes in cell morphology during wound closure.