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Generation of Transgenic Hydra by Embryo Microinjection

DOI :

10.3791/51888-v

September 11th, 2014

September 11th, 2014

13,704 Views

1Yale Stem Cell Center and Department of Cell Biology, Yale University School of Medicine, 2Department of Biological Chemistry and the Developmental Biology Center, University of California, Irvine

Stably transgenic Hydra are made by microinjection of plasmid DNA into embryos followed by random genomic integration and asexual propagation to establish a uniform line. Transgenic Hydra are used to track cell movements, overexpress genes, study promoter function, or knock down gene expression using RNAi.

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Transgenic Hydra

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