Measuring Endoplasmic Reticulum Stress and Unfolded Protein Response in HIV-1 Infected T-Cells and Analyzing its Role in HIV-1 Replication
1.1K Views
•
10:12 min
•
June 14th, 2024
DOI :
June 14th, 2024
•Chapters in this video
0:00
Introduction
1:30
Quantitation of HIV‐1 Infection in TZM‐bl Cells and Infection in CEM‐GFP T‐Cell Line
3:27
Thioflavin T Staining for Assessing Endoplasmic Reticulum Stress in CEM‐GFP Cells
5:13
Determining Activation and Expression of Unfolded Protein Response Markers in HIV‐1 Infected CEM‐GFP Cells
7:04
shRNA‐Mediated Gene Knockdown in HEK‐293T and Jurkat J6 Cells, Treatment with ER Stress Inducer and Analyzing its Effect on HIV‐1 Replication
Related Videos
11:10
Genotypic Inference of HIV-1 Tropism Using Population-based Sequencing of V3
12.2K Views
12:07
Preparation and Use of HIV-1 Infected Primary CD4+ T-Cells as Target Cells in Natural Killer Cell Cytotoxic Assays
25.9K Views
10:49
In vitro Uncoating of HIV-1 Cores
15.6K Views
09:26
In Vitro Assay to Evaluate the Impact of Immunoregulatory Pathways on HIV-specific CD4 T Cell Effector Function
14.0K Views
08:11
Assessing the Innate Sensing of HIV-1 Infected CD4+ T Cells by Plasmacytoid Dendritic Cells Using an Ex vivo Co-culture System.
8.6K Views
11:19
Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses
11.1K Views
12:03
Evaluation of the Efficacy And Toxicity of RNAs Targeting HIV-1 Production for Use in Gene or Drug Therapy
7.8K Views
10:34
Measurement of In Vitro Integration Activity of HIV-1 Preintegration Complexes
7.4K Views
09:38
Identification of Nucleolar Factors During HIV-1 Replication Through Rev Immunoprecipitation and Mass Spectrometry
7.9K Views
02:24
An Immunofluorescence-Based Method to Quantify Replication Stress in Cancer Cells
40 Views
Copyright © 2025 MyJoVE Corporation. All rights reserved