15.9K Views
•
20:59 min
•
September 23rd, 2009
DOI :
September 23rd, 2009
•0:00
Title
0:07
Introduction
0:43
End Repair of Sheared Genomic DNA
4:48
DNA-recovery by Gel Extraction and Ligation of Recovered DNA to the Fosmid Cloning Vector
20:32
Conclusion
2:10
Size-Selection of Genomic DNA by Pulsed-Field Gel Electrophoresis (PFGE)
10:18
Phage-packaging of Vector-insert Ligation Product and Infection of Phage-Resistant Bacteria
17:46
Colony Picking, Culturing, and Long-Term Storage
14:30
Plating and Titering
16:34
Preparation for Colony Picking
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