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15:57 min
October 9th, 2009
DOI :
10.3791/1529-v
Kapitel
0:00
Title
0:42
Introduction
1:11
Preparing the DNA Replication Template
2:44
Preparing Functionalized Coverslips
5:46
Assembling the Flow Chamber
10:03
Single-Molecule Replication Experiment
13:18
Single Molecule DNA Replication Data Analysis and Representative Results
15:00
Conclusion
Summary
This protocol demonstrates a simple single-molecule fluorescence microscopy technique for visualizing DNA replication by individual replisomes in real time.
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