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JoVE Journal

Biology

Author Produced

This content is Open Access.

Cell Electrofusion Visualized with Fluorescence Microscopy

14.0K Views

05:02 min

July 1st, 2010

July 1st, 2010

13,960 Views

0:00

Title

0:20

Loading the Cells with Cell Trackers CMFDA and CMRA

2:31

Electrofusion

3:39

Image Acquistion and Determination of the Fusion Yield

In this video we demonstrate efficient electrofusion of cells in vitro by means of modified adherence method using electroporation and the subsequent detection of fused cells visualization with fluorescence microscopy.

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