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08:21 min
June 6th, 2010
DOI :
10.3791/1878-v
Kapitel
0:00
Title
0:45
Introduction
1:11
siRNA Transfection and Cell Preparation
2:18
Microscope Setup and Image Acquisition
5:22
Image Processing and Analysis
7:21
Effect of Knockdown of Nucleoporin Nup153
7:48
Conclusion
Summary
Here we describe a basic protocol to image and quantify the mitotic timing of live mammalian tissue culture cells after siRNA transfection.
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