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Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus

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08:09 min

December 19th, 2013

December 19th, 2013

5,595 Views

0:05

Title

1:12

Cell Culture and Collection

2:32

Preparation of Tools and Cells for Microsurgery

3:43

High Resolution Imaging of Wound Healing in Live Stentor Cells

5:19

Analyzing Cytoplasmic Flow During Wound Healing

7:13

Results: Live Imaging of Stentor Cells Following Disruption

7:33

Conclusion

The giant ciliate Stentor coeruleus is a classical system for studying regeneration and wound healing in single cells. By imaging Stentor cells simultaneously at low and high magnification it is possible to measure cytoplasmic flows before, during, and after wounding.

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