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High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

DOI :

10.3791/50961-v

12:33 min

November 15th, 2013

November 15th, 2013

44,218 Views

1Natural Resource Ecology Laboratory, Colorado State University, 2Biosciences Division, Oak Ridge National Laboratory, 3Department of Bioengineering, University of Colorado

To measure potential rates of soil extracellular enzyme activities, synthetic substrates that are bound to a fluorescent dye are added to soil samples. Enzyme activity is measured as the fluorescent dye is released from the substrate by an enzyme-catalyzed reaction, where higher fluorescence indicates more substrate degradation.

Tags

High throughput

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