JoVE Journal

Environment

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High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities

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12:33 min

November 15th, 2013

November 15th, 2013

45,471 Views

0:05

Title

2:18

Assay Set-up

4:00

Incubation of Assay Plates

4:56

Fluorescent Measurements on a Microplate Fluorometer

5:43

Data Analysis

7:03

Results: Analysis of Extracellular Enzyme Activity (EEA)

11:05

Conclusion

To measure potential rates of soil extracellular enzyme activities, synthetic substrates that are bound to a fluorescent dye are added to soil samples. Enzyme activity is measured as the fluorescent dye is released from the substrate by an enzyme-catalyzed reaction, where higher fluorescence indicates more substrate degradation.

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