Fluorescent Measurements on a Microplate Fluorometer
5:43
Data Analysis
7:03
Results: Analysis of Extracellular Enzyme Activity (EEA)
11:05
Conclusion
To measure potential rates of soil extracellular enzyme activities, synthetic substrates that are bound to a fluorescent dye are added to soil samples. Enzyme activity is measured as the fluorescent dye is released from the substrate by an enzyme-catalyzed reaction, where higher fluorescence indicates more substrate degradation.