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JoVE Journal

Medicine

This content is Open Access.

Implementation of In Vitro Drug Resistance Assays: Maximizing the Potential for Uncovering Clinically Relevant Resistance Mechanisms

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08:46 min

December 9th, 2015

December 9th, 2015

10,392 Views

0:05

Title

1:01

Assessing GI50 for Compounds of Interest and Setting up Drug Resistance Assays

4:27

Isolating Single Cell Clones

6:37

Results: Assessment of Drug Resistance

8:06

Conclusion

Drug resistance to targeted therapeutics is widespread and the need to identify mechanisms of resistance--prior to or following clinical onset--is critical for guiding alternative clinical management strategies. Here, we present a protocol to couple derivation of drug-resistant lines in vitro with sequencing to expedite discovery of these mechanisms.

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