Results: Macromolecular Crowding of Fibroblasts and Keratinocytes
8:01
Conclusion
Transkript
The overall goal of this procedure is to enhance extracellular matrix deposition and improve on current 2D and 3D skin models using macromolecular crowding. Crowding improves the generation of cell-derived matrices, and mature organotypic cultures
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We present a protocol to obtain cell-derived matrices rich in extracellular matrix proteins, using macromolecular crowders (MMC). In addition, we present a protocol which incorporates MMC in 3D organotypic skin co-culture generation, which reduces culture time while maintaining maturity of construct.