Results: Velocity of Phagosome Migration in HIV-infected and Uninfected Human Macrophages
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Conclusion
Transkript
The overall goal of this analysis is to quantify phagosome velocity in HIV-1 infected macrophages using a simple manual tracking method. The advantage of this protocol is to provide an easy method to calculate the relative movements of groups of o
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We describe a method to measure the velocity of phagosomes moving towards the cell center in living cells infected with or without the human immunodeficiency virus (HIV) type 1, using spinning disk confocal fluorescence microscopy to identify fluorescent infected cells and bright field microscopy to detect phagosomes.