X-ray Fluorescence (XRF)

Überblick

Source: Laboratory of Dr. Lydia Finney — Argonne National Laboratory

X-ray fluorescence is an induced, emitted radiation that can be used to generate spectroscopic information. X-ray fluorescence microscopy is a non-destructive imaging technique that uses the induced fluorescence emission of metals to identify and quantify their spatial distribution.

Verfahren

1. Preparing the Silicon Nitride Windows

  1. Use reverse tweezers to pick up a window (silicon nitride windows will shatter if dropped).
  2. Place window onto a glass slide, flat side up.
  3. Adhere small pieces of Scotch tape to the sides of the window, and use these to adhere the windows to the bottom of the culture dish.
  4. Sterilize the windows in culture dishes with UV radiation. This can be accomplished with the auto-crosslink setting on a UV-crosslinking cabinet, followed by further UV i

Log in or to access full content. Learn more about your institution’s access to JoVE content here

Ergebnisse

The X-ray fluorescence map of an adherent cell is shown in Figure 1. Each panel shows the distribution of a particular element (e.g., copper, iron, zinc, etc) over the cell. The panel labeled 's_a' shows the absorption of X-rays.

Figure 1
Figure 1. X-ray fluorescence map of an adherent cell.

Log in or to access full content. Learn more about your institution’s access to JoVE content here

Anwendung und Zusammenfassung

X-ray fluorescence imaging can be a useful tool in many fields including geosciences, forensic science, materials science, biology, and even in studying our cultural heritage. In materials science, it can help find defects in chips and catalysts made with metals. In cultural heritage work, it has been used to identify poisonous metals in the hair of famous dead people (e.g., Beethoven), and to identify the source of paints used in art. In biology, it is used to study the natural metals that perform important biochemistry

Log in or to access full content. Learn more about your institution’s access to JoVE content here

Tags
X ray FluorescenceXRF SpectroscopyElemental AnalysisNon destructive TechniqueRoom Temperature AnalysisBiological SamplesForensic SamplesEnvironmental SamplesWorks Of ArtPowdersCrystalsLiquidsFluorescent RadiationFluorescence TechniqueX ray PhotonsInner Shell ElectronsSecondary Photon EmissionElement Identification

pringen zu...

0:00

Overview

1:07

Principles of X-ray Fluorescence

2:37

Preparing the Silicon Nitride Windows

3:09

Plating and Fixing Cells on the Windows

4:10

Generating X-ray Fluorescence Images

5:39

Representative Results

6:09

Applications

7:26

Summary

Videos aus dieser Sammlung:

article

Now Playing

X-ray Fluorescence (XRF)

Analytical Chemistry

25.3K Ansichten

article

Probenvorbereitung für die analytische Charakterisierung

Analytical Chemistry

83.7K Ansichten

article

Interne Standards

Analytical Chemistry

203.4K Ansichten

article

Standard-Additionsverfahren

Analytical Chemistry

318.8K Ansichten

article

Kalibrierkurven

Analytical Chemistry

789.1K Ansichten

article

UV/Vis-Spektroskopie

Analytical Chemistry

616.5K Ansichten

article

Raman-Spektroskopie für die chemische Analyse

Analytical Chemistry

50.8K Ansichten

article

Gaschromatographie (GC) mit Flammen-Ionisations-Detektion

Analytical Chemistry

279.9K Ansichten

article

Hochleistungs-Flüssigkeitschromatographie (HPLC)

Analytical Chemistry

381.8K Ansichten

article

Ionenaustausch-Chromatographie

Analytical Chemistry

263.0K Ansichten

article

Kapillarelektrophorese (CE)

Analytical Chemistry

93.0K Ansichten

article

Einführung in die Massenspektrometrie

Analytical Chemistry

111.4K Ansichten

article

Rasterelektronenmikroskopie (SEM)

Analytical Chemistry

86.6K Ansichten

article

Elektrochemische Messungen von Trägerkatalysatoren mit einem Potentiostat / Galvanostat

Analytical Chemistry

51.2K Ansichten

article

Zyklische Voltammetrie (CV)

Analytical Chemistry

123.4K Ansichten

JoVE Logo

Datenschutz

Nutzungsbedingungen

Richtlinien

Forschung

Lehre

ÜBER JoVE

Copyright © 2025 MyJoVE Corporation. Alle Rechte vorbehalten