Stress Granule Formation Indication and Immunofluorescent Analysis
6:09
Fluorescence Imaging Analysis
8:47
Results: Representative Stress Granule Analysis
10:20
Conclusion
Transkript
The overall goal of this experiment is to assess the effects of a bacterial infection on the ability of host cells to form stress granules to response to exogenous stress. This method is a valuable tool in the field of cellular microbiology for as
Sign in or start your free trial to access this content
We describe a method for the qualitative and quantitative analysis of stress granule formation in mammalian cells after the cells are challenged with bacteria and a number of different stresses. This protocol can be applied to investigate the cellular stress granule response in a wide range of host-bacterial interactions.