T Cell Culture Preparation and CD4+ T Cell Isolation
3:38
Th17 Polarization
4:41
Electroporation of In Vitro Polarized Human Th17 Cells
8:03
Results: Robust In Vitro Differentiated Human Th17 Cell Cultures
9:48
Conclusion
Transkript
The overall goal of this procedure is to efficiently transfect primary human T cells with small RNA reagents using next generation electroporation. This method can help answer key questions in T cell biology, such as how specific gene products reg
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Next generation electroporation is an efficient method for transfecting human Th17 cells with small RNAs to alter gene expression and cell behavior.