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JoVE Journal

Medicine

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Semi-automated Analysis of Mouse Skeletal Muscle Morphology and Fiber-type Composition

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08:36 min

August 31st, 2017

August 31st, 2017

10,192 Views

0:05

Title

0:41

Day 1: Primary Immunostaining of Mouse Muscle Sections

1:57

Day 2: Secondary Immunostaining of Mouse Muscle Sections

3:24

Collecting Muscle Images and their Analysis with the Fiber Quantification Macro

6:23

Results: Representative Immunostaining and Morphometric Analysis

7:50

Conclusion

Transkript

The overall goal of this protocol is to use state-of-the-art immunohistochemistry to accurately and rapidly identify skeletal muscle fiber types. This method can help answer key questions in the field of muscle biology such as whether fiber type c

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Immunohistochemical staining of myosin heavy chain isoforms has emerged as the state-of-the-art discriminator of skeletal muscle fiber-type (i.e., type I, type IIA, type IIX, type IIB). Here, we present a staining protocol along with a novel semi-automated algorithm that facilitates rapid assessment of fiber-type and fiber morphology.

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