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JoVE Journal

Biochemistry

This content is Free Access.

Production of siRNA-Loaded Lipid Nanoparticles using a Microfluidic Device

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06:02 min

March 22nd, 2022

March 22nd, 2022

8,142 Views

0:04

Introduction

0:54

Preparation of Lipid Solutions and the siRNA/ Buffer Solution

1:58

Set-Up of the iLiNP Device and Production of LNPs

2:35

Dialysis of the LNP Suspension and LNP Size Measurement

3:24

Results: POPC LNP Size Distributions Produced by the iLiNP Device at Different Flow Rate Ratios and Characterization and Cytotoxicity of siRNA-Loaded LNPs

5:22

Conclusion

Transkript

Microfluidic-based lipid nanoparticle (LNP) production methods have attracted attention in drug delivery systems (DDSs), including RNA delivery. This protocol describes the fabrication, LNP (siRNA-loaded LNP) production, and LNP evaluation processes using our original microfluidic device named iLiNP.

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