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Neuroscience

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Isolation, Culture, and Characterization of Primary Schwann Cells, Keratinocytes, and Fibroblasts from Human Foreskin

5.3K Views

10:36 min

March 23rd, 2022

March 23rd, 2022

5,331 Views

0:04

Introduction

1:17

Epidermis and Dermis Separation Procedure

3:03

Keratinocyte Isolation from the Epidermis

5:02

Isolation of Schwann Cells (SCs) and Fibroblast from the Dermis

8:18

Results: Characterization of Human Foreskin-Derived Primary Keratinocytes, Schwann Cells, and Fibroblasts

9:43

Conclusion

Transkript

The skin contains multiple cell types including, keratinocytes, fibroblasts, and nerve Schwann cells. This protocol allows isolation of each of these cell types for in vitro experiments. Isolation and establishment of individual primary cell cultu

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The study of wound healing associated with musculoskeletal injury often requires the assessment of in vitro interactions between Schwann cells (SCs), keratinocytes, and fibroblasts. This protocol describes the isolation, culturing, and characterization of these primary cells from the human foreskin.

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