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Fox Chase Cancer Center

12 ARTICLES PUBLISHED IN JoVE

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Biology

Dendra2 Photoswitching through the Mammary Imaging Window
Bojana Gligorijevic 1,2, Dmitriy Kedrin 1, Jeffrey E Segall 1, John Condeelis 1,2, Jacco van Rheenen 1,2,3
1Department of Anatomy and Structural Biology, Albert Einstein College of Medicine - Yeshiva University, 2Gruss Lipper Biophotonics Center, Albert Einstein College of Medicine - Yeshiva University, 3Hubrecht Institute-KNAW and University Medical Center Utrecht

Intravital photoswitching and tracking of Dendra2-labeled tumor cells through the Mammary Imaging Window is a technique which allows us to image the metastatic behavior of tumor cells in chosen tumor microenvironments over a timescale of days.

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Biology

In vivo Imaging and Therapeutic Treatments in an Orthotopic Mouse Model of Ovarian Cancer
Alexis B. Cordero 1, Youngjoo Kwon 1, Xiang Hua 2, Andrew K. Godwin 1
1Department of Medical Oncology, Women's Cancer Program, 2Transgenic Mouse Facility, Fox Chase Cancer Center

Orthotopic animal models of ovarian cancer replicate better human disease and therefore enhance our understanding of cancer progression and tumor response to therapy. A mouse model receives an intrabursal injection of luciferase-expressing ovarian tumor cells. Treatment is administered via oral gavage. Tumor growth is monitored by in vivo imaging system.

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Immunology and Infection

An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses
Selene Nunez-Cruz 1, Denise C. Connolly 2, Nathalie Scholler 1
1Penn Ovarian Cancer Research Center, Center for Research on Reproduction and Womans Health, Department of Obstetrics and Gynecology, University of Pennsylvania-School of Medicine, 2Women's Cancer Program, Fox Chase Cancer Center

To study in vivo tumor growth and tumor microenvironment, we used a syngeneic and orthotopic mouse model of ovarian cancer in immunocompetent animals. We transduced a mouse tumor cell line (MOV1) with Katushka fluorescent protein (MOV1KAT) and here we show its orthotopic implantation in ovary and in vivo imaging.

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Neuroscience

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae
Diana Zitserman 1, Fabrice Roegiers 1
1Epigenetics and Progenitor Cells Keystone, Fox Chase Cancer Center

In this video, we describe a method for live cell imaging of asymmetrically dividing sensory organ progenitor cells and epidermal cells in intact Drosophila pupae

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Medicine

An in vivo Assay to Test Blood Vessel Permeability
Maria Radu 1, Jonathan Chernoff 1
1Fox Chase Cancer Center

We are presenting an in vivo assay to test blood vessel permeability. This assay is based on intravenous injection of a dye and subsequent visualization of its diffusion into interstitial spaces.

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Medicine

Carotid Artery Infusions for Pharmacokinetic and Pharmacodynamic Analysis of Taxanes in Mice
Joely D. Jacobs 1, Elizabeth A. Hopper-Borge 1
1Program in Developmental Therapeutics, Fox Chase Cancer Center

This method was developed with the goal of delivering a steady drug solution via the carotid artery, to assess the pharmacokinetics of novel drugs in mouse models.

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Biochemistry

Detection of Heterodimerization of Protein Isoforms Using an in Situ Proximity Ligation Assay
Sofiia Karchugina 1, Jonathan Chernoff 1
1Fox Chase Cancer Center

Here, we show how to use a Proximity Ligation Assay (PLA) to visualize MST1/MST2 heterodimerization in fixed cells with high sensitivity.

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Cancer Research

Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia
Daniela Di Marcantonio 1, Stephen M. Sykes 1
1Blood Cell Development and Function Program, Fox Chase Cancer Center

We describe a method for using multiparameter flow cytometry to detect mitochondrial reactive oxygen species (ROS) in murine healthy hematopoietic stem and progenitor cells (HSPCs) and leukemia cells from a mouse model of acute myeloid leukemia (AML) driven by MLL-AF9.

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Biology

Time-Resolved Fluorescence Imaging and Analysis of Cancer Cell Invasion in the 3D Spheroid Model
Louisiane Perrin 1, Theodore Tucker 1, Bojana Gligorijevic 1,2
1Bioengineering Department, Temple University, 2Cancer Biology Program, Fox Chase Cancer Center

Presented here is a protocol for the fabrication of a spheroid imaging device. This device enables dynamic or longitudinal fluorescence imaging of cancer cell spheroids. The protocol also offers a simple image processing procedure for the analysis of cancer cell invasion.

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Cancer Research

Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids
Samuel Flashner *1, Cecilia Martin *1,2, Norihiro Matsuura 1, Masataka Shimonosono 1, Yasuto Tomita 1, Masaki Morimoto 1, Ogoegbunam Okolo 1, Victoria X. Yu 1,3, Anuraag S. Parikh 1,3, Andres J. P. Klein-Szanto 4, Kelley Yan 1,2, Joel T. Gabre 1,5, Chao Lu 1,6, Fatemeh Momen-Heravi 1,7, Anil K. Rustgi 1,5, Hiroshi Nakagawa 1,2,5
1Herbert Irving Comprehensive Cancer Center, Columbia University, 2Organoid and Cell Culture Core, Columbia University Digestive and Liver Diseases Research Center, Columbia University, 3Department of Otolaryngology, Head and Neck Surgery, Columbia University, 4Histopathology Facility, Fox Chase Cancer Center, 5Division of Digestive and Liver Diseases, Department of Medicine, Columbia University, 6Department of Genetics and Development, Columbia University, 7Section of Oral, Diagnostic and Rehabilitation Sciences, College of Dental Medicine, Columbia University

This protocol describes the key steps to generate and characterize murine oral-esophageal 3D organoids that represent normal, preneoplastic, and squamous cell carcinoma lesions induced via chemical carcinogenesis.

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Cancer Research

Tracking Tumor Cell Dissemination from Lung Metastases Using Photoconversion
Madeline Friedman-DeLuca 1,2, Prachiben P. Patel 1,2, Burcu Karadal-Ferrena 1,2, Nicole D. Barth 1,2, Camille L. Duran 1,2, Xianjun Ye 1,2,3, Michael Papanicolaou 2,4, John S. Condeelis 2,3,4,5,6,7, Maja H. Oktay 1,2,3,5,6,7, Lucia Borriello *1,2,8, David Entenberg *1,2,3,5,6
1Department of Pathology, Albert Einstein College of Medicine/Montefiore Medical Center, 2Cancer Dormancy and Tumor Microenvironment Institute, Albert Einstein College of Medicine/Montefiore Medical Center, 3Gruss-Lipper Biophotonics Center, Albert Einstein College of Medicine/Montefiore Medical Center, 4Department of Cell Biology, Albert Einstein College of Medicine/Montefiore Medical Center, 5Montefiore Einstein Cancer Center, Albert Einstein College of Medicine/Montefiore Medical Center, 6Integrated Imaging Program for Cancer Research, Albert Einstein College of Medicine/Montefiore Medical Center, 7Department of Surgery, Albert Einstein College of Medicine/Montefiore Medical Center, 8Department of Cancer and Cellular Biology, Lewis Katz School of Medicine, Fox Chase Cancer Center

We present a method for studying tumor cell redissemination from lung metastases involving a surgical protocol for the selective photoconversion of lung metastases, followed by the identification of redisseminated tumor cells in tertiary organs.

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Cancer Research

A Simple, Rapid, and Effective Method for Tumor Xenotransplantation Analysis in Transparent Zebrafish Embryos
Monika Verma 1, Michele Rhodes 1, Susan Shinton 1, David L. Wiest 1
1Nuclear Dynamics and Cancer Program, Fox Chase Cancer Center

We describe a protocol for xenotransplantation into the yolk of transparent zebrafish embryos that is optimized by a simple, rapid staging method. Post-injection analyses include survival and assessing the disease burden of xenotransplanted cells by flow cytometry.

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