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Application of Stopped-flow Kinetics Methods to Investigate the Mechanism of Action of a DNA Repair Protein

18.0K Views

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11:01 min

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March 31st, 2010

DOI :

10.3791/1874-v

March 31st, 2010

•
18,014 Views
1Molecular Biology and Biochemistry Department, Wesleyan University

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Stopped flow Kinetics Methods
Mechanism Of Action
DNA Repair Protein
Transient Kinetic Analysis
Biological Macromolecules
Active Site Concentrations
Intrinsic Rate Constants
Macromolecular Function
Enzymes
Pre steady State Measurements
Reaction Pathway
Steady State Measurements
Catalytic Efficiency
Catalytic Specificity
Protein protein Interactions
Protein ligand Interactions
Rate limiting Conformational Changes
Millisecond Timescale
Stopped flow Method
Chemical quench Flow Methods
Optical Signal
Fluorescence
Substrate Binding
Product Release
Catalytic Turnover
Msh2 Msh6 Protein
Eukaryotic DNA Repair Protein
Base pair Mismatches
Insertion deletion Loops In DNA
Mismatch Repair MMR
Genomic Integrity
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Application of Stopped-flow Kinetics Methods to Investigate the Mechanism of Action of a DNA Repair Protein

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