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GF Artemia nauplii Preparation for Chronic GF Zebrafish Nutrition

33 Views

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01:24 min

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April 12th, 2024

DOI :

10.3791/201491-v

April 12th, 2024

•

33 Views

Pan-Pan Jia¹, Xiang-Ru Liu¹, Ming-Fei Wu¹, Yan Li¹, Lan-Chen Zhang¹, De-Sheng Pei¹

¹School of Public Health, Chongqing Medical University

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Germ free GF Artemia Nauplii Preparation For Chronic GF Zebrafish Nutrition 1 Obtain 0 25 Grams Of Artemia Cysts And Rinse Them With 2 4 Grams Per Liter Sodium Hypochlorite For 5 Minutes 2 Filter The Rinsed Cysts With A Sterilized Ultra dense Filter And Wash With Sterilized Ultrapure Water For 1 2 Minutes 3 After 3 Washes

Transfer The Cysts To 100 Milliliters Of 2 5 Sterile Saline And Mix Well 4 Pack 50 Milliliters Of The Treated Artemia Cyst Mix Into A Sterile 100 milliliter Bottle

Seal It Thoroughly With A Film

And Place It In A Shaking Incubator With Light 5 Extract 30 Milliliters Of Hatching Artemia Nauplii From The Middle And Lower Layers Using A Pasteur Pipette 6 Filter And Wash The Artemia 3 Times With Sterile Ultrapure Water For About 1 Minute

And Add 4 Milliliters Of Sterile Ultrapure Water 7 Retrieve Actively Swimming Artemia From The Upper Layer Using A Disposable Pasteur Pipette

And Prepare The Solution For Feeding 8 Microscopic Observation Co

From the series

Optimizing GF Zebrafish Models: Generation, Maintenance, and Microbial Studies

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