January 3rd, 2012
•An in vitro model for cerebral malaria sequestration is described1. Plasmodium falciparum infected red blood cells are selected for binding to immortalized human brain microvascular endothelial cells. The selected parasites show a distinct phenotype. The selection process can be applied using various P. falciparum strains and endothelial cell lines.
Videos relacionados
An In vitro Co-infection Model to Study Plasmodium falciparum-HIV-1 Interactions in Human Primary Monocyte-derived Immune Cells
A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting
Isolation and Analysis of Brain-sequestered Leukocytes from Plasmodium berghei ANKA-infected Mice
Separation of Plasmodium falciparum Late Stage-infected Erythrocytes by Magnetic Means
Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions
Isolation of Myeloid Dendritic Cells and Epithelial Cells from Human Thymus
High Yield Purification of Plasmodium falciparum Merozoites For Use in Opsonizing Antibody Assays
Methods to Investigate the Regulatory Role of Small RNAs and Ribosomal Occupancy of Plasmodium falciparum
Overexpression and Purification of Human Cis-prenyltransferase in Escherichia coli
Pneumococcus Infection of Primary Human Endothelial Cells in Constant Flow
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados