The c-FOS Protein Immunohistological Detection: A Useful Tool As a Marker of Central Pathways Involved in Specific Physiological Responses In Vivo and Ex Vivo
Day 1 Endogenous Peroxidase Activity Destruction, Non-specific Binding Site Blockade and Incubation with the Primary Antibody
1:44
Day 3 Incubation with Secondary Antibody and Development of a Color Reaction
3:21
Sample Mounting
4:16
Results: c-FOS-positive Neurons on Sections of the Medulla Oblongata in Control Mice and Mice Submitted to Hypoxia In Vivo
5:17
Conclusion
Transcribir
The overall goal of this immunohistochemical detection against c-FOS protein is to assess the neuronal activity in brain tissues. This method can help answer our key questions in the neurophysological field such as brain mapping and identification
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Here, we present a protocol based on c-FOS protein immunohistological detection, a classical technique used for the identification of neuronal populations involved in specific physiological responses in vivo and ex vivo.