Results: Measuring DNA Strand Breaks and Oxidative DNA Damage Using the Alkaline Comet Assay
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Conclusion
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The overall goal of the in vivo alkaline comet assay and the enzyme-modified alkaline comet assay, is to concurrently measure direct and oxidative DNA damage at the single-cell level. This method was first proposed as a regulatory tool for safety
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The alkaline comet assay measures DNA strand breaks in eukaryotic cells. By adding an Endonuclease III or human 8-oxoguanine-DNA-N-glycosylase digestion step, the assay can efficiently detect oxidative DNA damage. We describe methods for using these assays to detect DNA damage in rat liver.