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piggyBac Transposon System Modification of Primary Human T Cells

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10:02 min

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November 5th, 2012

DOI :

November 5th, 2012

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17,050 Views

Sunandan Saha¹^,², Yozo Nakazawa³, Leslie E. Huye⁴^,⁵, Joseph E. Doherty²^,⁶, Daniel L. Galvan², Cliona M. Rooney⁴^,⁵^,⁷, Matthew H. Wilson¹^,²^,⁴^,⁸

¹Program in Translational Biology and Molecular Medicine, Baylor College of Medicine , ²Department of Medicine, Division of Nephrology, Baylor College of Medicine , ³Department of Immunology and Pathology, Shinshu University School of Medicine, ⁴Center for Cell and Gene Therapy, Baylor College of Medicine , ⁵Department of Pediatrics, Baylor College of Medicine , ⁶Program in Cell and Molecular Biology, Baylor College of Medicine , ⁷Department of Molecular Virology and Microbiology, Baylor College of Medicine , ⁸Michael E. DeBakey VA Medical Center

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PiggyBac Transposon System

Primary Human T Cells

Transposase Enzyme

Inverted Repeat Elements

Adoptive Immunotherapy

Non viral Methodology

Genetic Modification

Inducible Suicide Gene

Genomic Integration Sites

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