Burst Integration Fluorescence Lifetime Analysis of Standards
7:34
dsDNA and Protein Sample Measurements and Analysis
9:28
Results: Inter-dye Distance Determination in Single Molecules
10:54
Conclusion
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The overall goal of this protocol is to perform high precision inter-dye distance measurements using multi-parameter fluorescence detection of F
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A protocol for high-precision FRET experiments at the single molecule level is presented here. Additionally, this methodology can be used to identify three conformational states in the ligand-binding domain of the N-methyl-D-aspartate (NMDA) receptor. Determining precise distances is the first step towards building structural models based on FRET experiments.