Single Cell Preparation, Flow Cytometry, and Plating
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Results: Effect of siRNA Medicated Knockdown of Apc in Lgr5+ Cells
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Conclusion
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The overall goal of the organoid reconstitution assay is the functional analysis of the two major components of the intestinal stem cell niche Lgr5 positive in the Paneth cells through their genetic or biochemical modification followed by reconsti
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Intestinal organoid cultures are established from whole crypts and do not allow the analysis of self-renewal and differentiation in a cell-specific fashion. This protocol describes reconstitution of sorted stem (Lgr5+) and niche (Paneth) cells, which give rise to organoids while enabling their prior biochemical and genetic modification and functional analysis.