The overall goal of this protocol is prepare meiotic chromosome spreads from mouse spermatocytes to allow the study of male meiosis. This method can help answer key questions in the reproductive biology field about the mechanisms that regulate mei
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Meiosis is the developmental process by which gametes are formed through a single round of DNA replication and two successive rounds of chromosome segregation. Mammalian meiosis can be examined by utilizing a technique to prepare meiotic chromosome spreads. Here, we demonstrate a method of preparing surface-spread nuclei from mouse spermatocytes.