February 12th, 2018
•The goal of this protocol is to obtain high viability and high yield of hepatocytes and sinusoidal endothelial cells from liver. This is accomplished by perfusing the liver with a type IV collagenase solution via the portal vein, followed by differential centrifugation to obtain hepatocytes and sinusoidal endothelial cells.
Videos relacionados
Tandem Affinity Purification of Protein Complexes from Eukaryotic Cells
Purification of Biotinylated Cell Surface Proteins from Rhipicephalus microplus Epithelial Gut Cells
Dynamic Proteomic and miRNA Analysis of Polysomes from Isolated Mouse Heart After Langendorff Perfusion
Isolation of Tissue Extracellular Vesicles from the Liver
Translating Ribosome Affinity Purification (TRAP) for RNA Isolation from Endothelial Cells In Vivo
SUMO-Binding Entities (SUBEs) as Tools for the Enrichment, Isolation, Identification, and Characterization of the SUMO Proteome in Liver Cancer
Cell Fractionation of U937 Cells by Isopycnic Density Gradient Purification
Measurement of Fatty Acid β-Oxidation in a Suspension of Freshly Isolated Mouse Hepatocytes
Ex Vivo Hepatic Perfusion Through the Portal Vein in Mouse
Extraction and Purification of FAHD1 Protein from Swine Kidney and Mouse Liver
ACERCA DE JoVE
Copyright © 2024 MyJoVE Corporation. Todos los derechos reservados