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JoVE Journal

Cancer Research

This content is Open Access.

Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts

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07:18 min

June 13th, 2019

June 13th, 2019

6,727 Views

0:04

Title

0:44

Sarcoma Tissue Sample Processing and Patient-derived Xenograft (PDX) Formation

2:32

Tumor-initiating Cell (TIC) Sorting and Sarcosphere Formation

5:11

Results: Representative Human Leukocyte Antigen (HLA)-1-Negative TIC Characterization

6:42

Conclusion

Transcribir

We describe a detailed protocol for the isolation of tumor-initiating cells from human sarcoma patient-derived xenografts by fluorescence-activated cell sorting, using human leukocyte antigen-1 (HLA-1) as a negative marker, and for the further validation and characterization of these HLA-1-negative tumor-initiating cells.

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