Stereotaxic Surgery for Genetic Manipulation in Striatal Cells of Neonatal Mouse Brains

14.4K Views

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09:44 min

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July 10th, 2018

DOI :

10.3791/57270-v

July 10th, 2018

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14,380 Views

Shih-Yun Chen¹, Hsiao-Ying Kuo¹, Fu-Chin Liu¹^,²

¹Institute of Neuroscience, National Yang-Ming University, ²Brain Research Center, National Yang-Ming University

Capítulos

0:04

Title

0:40

Preparing the Microinjection Apparatus

4:09

Microinjecting Neonatal Mice

7:30

Results: Analysis of Injection of Cre-expressing Virus into P2 Striatum

9:03

Conclusion

Transcribir

This method can help answer key questions in the genetic and neuro development such as during postnatal development. The main advantage of this technique is that it is a simple technique with home-made device. Visual demonstration of this method i

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Resumen

We describe a protocol of stereotaxic surgery with a homemade head-fixed device for microinjecting reagents into the striatum of neonatal mouse brains. This technique allows genetic manipulation in neuronal cells of specific regions of neonatal mouse brains.

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